基于均匀设计法的钩吻配伍玉叶金花的毒性作用规律研究

Uniform Design Research on the Compatibility Toxicity of Gelsemium Elegans Benth. and Mussaenda Pubescens

关键词:  均匀设计  急性毒性  玉叶金花  钩吻

Key words:  uniform design  acute toxicity  Mussaenda pubescens  Gelsemium elegans Benth.

摘要:

目的 探讨钩吻配伍玉叶金花后的毒性变化规律。方法 采用U₇^*(7⁴)均匀设计表，按2因素7水平设计配比实验，以小鼠死亡率为考察指标进行急性毒性评价，并考察钩吻配伍前后的LD₅₀。结果 均匀设计得到的多元函数模型具有显著性统计学意义(P<0.05)，针对函数模型求偏导得最佳配比0.54∶14.23(钩吻∶玉叶金花)，三维曲面分析的低毒性的剂量配比范围(钩吻∶玉叶金花= 1∶22~36)；钩吻LD50为0.32 g·kg^-1，而配伍后LD50为0.68 g·kg^-1，毒性大幅降低。结论 钩吻配伍玉叶金花后毒性降低，可为临床合理用药提供参考依据。

Abstract:

OBJECTIVE To study the toxicity variation of Gelsemium elegans Benth. combined with Mussaenda pubescens. METHODS The U₇^*(7⁴) uniform design method was used by two factors and seven levels to investigate the toxicity changes with the death of mice as index in different groups of Gelsemium elegans Benth. combined with Mussaenda pubescens. Then LD₅₀ of Gelsemium elegans Benth. combined with Mussaenda pubescens were determinated. RESULTS The multivariate function model of uniform design was statistically significant(P<0.05). The best ratio of Gelsemium elegans Benth. combined with Mussaenda pubescens was 0.54∶14.23 by partial derivative. The dose ratio range from 1∶22 to 1∶36 by 3D surface analysis. The LD₅₀ of Gelsemium elegans Benth. was 0.32 g·kg^-1, and the LD50 was of Gelsemium elegans Benth combined with Mussaenda pubescens was 0.68 g·kg^-1. The toxicity was greatly reduced. CONCLUSION The toxicity of Gelsemium elegans Benth. combined with Mussaenda pubescens reduce. The results offer scientific basis for reasonable usage.