响应面法优化超声波辅助提取板蓝根中黄酮工艺及其抗氧化活性研究

Optimization of Extracting Flavonoids from Isatidis Radix by Response Surface Methodology and Its Antioxidant Activity

关键词:  板蓝根  黄酮  响应面法  优化  抗氧化

Key words:  Isatidis Radix  flavonoids  response surface analysis  optimization  antioxidant

摘要:

目的 确定板蓝根黄酮的最佳提取工艺，并初步评价其抗氧化活性。方法 在单因素实验基础上，利用响应面法优化板蓝根总黄酮的提取条件; 通过总黄酮对1，1-二苯基-2-苦基肼自由基的清除作用来研究其抗氧化活性。结果 板蓝根黄酮最佳提取工艺为料液比1∶24.1、提取时间29 min和乙醇浓度60%，此条件下黄酮得率为54.4 mg·g^-1；板蓝根黄酮对1，1-二苯基-2-苦基肼自由基具有较好的清除能力。结论 该优化工艺实现了板蓝根中黄酮类化合物的高效提取。

Abstract:

OBJECTIVE To explore the optimum extraction process of flavonoids from Isatidis Radix and evaluate its antioxidant activity. METHODS Based on the single-factor tests, the extraction conditions of total flavonoids from Isatidis Radix were optimized by response surface methodology. Meanwhile, the antioxidant activity of flavonoids was assessed by scavenging assays of 1,1-diphenyl-2-picrylhydrazyl(DPPH). RESULTS The optimum extraction conditions were as follows: ethanol concentration was 60%, liquid-solid ratio was 1∶24.1, and extraction time was 29 min. Under the optimal conditions, the extraction rate of total flavonoids was 54.4 mg·g^-1. DPPH radical scavenging capacity of flavonoids was good. CONCLUSION The target of extracting flavonoids compound efficiently from Isatidis Radix has been achieved by the optimized technology.