虫草多糖对荷瘤小鼠T淋巴细胞及其亚群数量与功能的影响

Effects of Polysaccharides of Cordyceps Sinensis Mycelium on the Number and Function of T Lymphocytes in Tumor-bearing Mice

关键词:  冬虫夏草菌丝体  虫草多糖  T淋巴细胞亚群  S180肉瘤

Key words:  Cordyceps sinensis Mycelium  cordyceps polysaccharide  T lymphocyte subgroup  S180 sarcoma

摘要:

目的 研究虫草多糖对荷瘤小鼠T淋巴细胞功能的影响。方法 除正常对照组外，其余小鼠右前肢腋窝皮下注射S180肉瘤细胞悬液建立小鼠荷瘤模型。荷瘤小鼠随机分为荷瘤对照(等容生理盐水)组、香菇多糖(1 mg·kg^-1)组、虫草多糖高、中、低剂量(200，100，50 mg·kg^-1)组，腹腔注射，每天1次，连续13 d后，检测虫草多糖对荷瘤小鼠的抑瘤作用以及荷瘤小鼠外周血T淋巴细胞及其亚群数量、Th1/Th2类细胞因子水平和脾淋巴细胞转化功能。结果 虫草多糖高、中剂量组可明显抑制S180肉瘤生长，提高荷瘤小鼠脾脏重量和脾脏系数；提高外周血CD3⁺细胞(总T淋巴细胞)、CD4⁺CD8^-亚群数量及CD4⁺CD8^-/CD4^-CD8⁺比值，提高Th1淋巴细胞分泌的细胞因子TNF-α和IL-2水平，并显著促进脾脏T淋巴细胞增殖转化能力。结论 虫草多糖具有明显的抗肿瘤活性，其机制可能与调节机体T淋巴细胞及其亚群的数量与分泌功能有关。

Abstract:

OBJECTIVE To study the effects of polysaccharides of Cordyceps sinensis Mycelium (cordyceps polysaccharide) on the function of T lymphocytes in tumor-bearing mice. METHODS Tumor-bearing mice were established by inoculated with S180 cells. The tumor-bearing mice were randomly divided into the tumor bearing group (physiological saline), lentinan group(1 mg·kg^-1), cordyceps polysaccharide groups in high, medium and low dose(200, 100, 50 mg·kg^-1). After celiac injection of cordyceps polysaccharide for 13 d (once a day), the number of T lymphocytes and its subgroups in peripheral blood, Th1/Th2 cytokines and transformation rates of spleen T lymphocytes were detected; and the thymus index, the spleen index and the inhibition rate of tumor were measured. RESULTS High and medium dose of cordyceps polysaccharide groups had significantly reduced growth of S180 sarcoma, increased spleen weight and spleen coefficient of tumor-bearing mice, increased the number of CD3⁺ cells (total T lymphocytes), CD4⁺CD8^- subgroup and CD4⁺CD8^-/CD4^-CD8⁺ ratio in peripheral blood, increased the level of TNF-α and IL-2 in Th1 lymphocytes, and significantly increased proliferation and transformation of spleen T lymphocytes. CONCLUSION Cordyceps polysaccharide has antitumor activity in vivo, and the underlying mechanism may relate to the regulation of the number and the immune function of T lymphocytes and its subgroup by cordyceps polysaccharide.