| 引用本文: | 荆彦民,马瑞丽.HPLC同时测定大血藤中绿原酸、咖啡酸和香草酸的含量[J].中国现代应用药学,2016,33(5):592-595. |
| JING Yanmin,MA Ruili.Simultaneous Determination of Chlorogenic Acid, Caffeic Acid and Vanillic Acid in Sargentodoxae Caulis by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(5):592-595. |
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| 摘要: |
| 目的 建立同时测定大血藤各部位绿原酸、咖啡酸和香草酸含量的高效液相色谱方法。方法 色谱柱为Kromasil C18柱(4.6 mm×250 mm，4.5 μm)，流动相：A为1%冰醋酸，B为甲醇，以A∶B=68∶32等度洗脱；柱温：30 ℃；流速：1 mL·min-1；检测波长：327 nm；进样量：20 μL。结果 在测定样品中，绿原酸和咖啡酸在乙酸乙酯部位含量最高，分别为4.52%，0.11%，香草酸在二氯甲烷部位含量最高，为5.8%。结论 该方法简便、准确、重复性高，可用于大血藤中绿原酸、咖啡酸、香草酸含量的测定。 |
| 关键词: 高效液相色谱法 大血藤 绿原酸 咖啡酸 香草酸 |
| DOI： |
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| 基金项目:甘肃省定西市道地中药材产业化推广及惠民示范工程(S2013GMG100004) |
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| Simultaneous Determination of Chlorogenic Acid, Caffeic Acid and Vanillic Acid in Sargentodoxae Caulis by HPLC |
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JING Yanmin,MA Ruili
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1.Dingxi Academy of Agricultural Sciences, Dingxi 743000, China;2.School of Pharmacy, Jiangsu University, Zhenjiang 212013, China
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| Abstract: |
| OBJECTIVE To develop an HPLC method for simultaneous determination of chlorogenic acid, caffeic acid and vanillic acid in each fraction of Sargentodoxae Caulis. METHODS The analysis was carried on a column of Kromasil C18(4.6 mm×250 mm, 4.5 μm). Isocratic elution, mobile phase consisted of solvent A (1%, solution of HAc in water) and solvent B (methanol) at the ratio of 68∶32, the flow rate was 1.0 mL.min-1, the injection volume was 20 μL, the detection wavelength was 327 nm, the column temperature was at 30 ℃. RESULTS Ethyl acetate fraction had the highest concentration of chlorogenic acid (4.52%) and caffeic acid (0.11%) and dichloromethane fraction possessed the highest concentration of vanillic acid of 5.8%. CONCLUSION This method is simple, accurate, rapid and suitable for the usual quality control of Sargentodoxae Caulis. |
| Key words: HPLC Sargentodoxae Caulis chlorogenic acid caffeic acid vanillic acid |
