疏肝调脂方对高脂血症模型大鼠血脂、肝肾功能及微循环的影响

Effect of Shugan Tiaozhi Fang on Blood Lipid, Liver and Kidney Function and Microcirculation in Hyperlipidemia Model Rats

关键词:  疏肝调脂方  高脂血症  血脂  微循环

Key words:  Shugan Tiaozhi Fang  hyperlipidemia  blood lipid  microcirculation

摘要:

目的 探讨疏肝调脂方对高脂血症模型大鼠血脂、肝肾功能及微循环的影响。方法 采用高糖高脂饲料复合酒饮建立高脂血症大鼠模型，造模2周后大鼠灌胃给予疏肝调脂方(0.66，1.32，2.64 g·kg^-1)，每天1次，连续6周。观察给药前后各组大鼠的一般体征；测定血清总胆固醇(total cholesterol，TC)、甘油三酯(triglyceride，TG)、高密度脂蛋白胆固醇(high density lipoprotein-cholesterol，HDL-C)、肌酐(creatinine，CR)、尿素氮(blood urea nitrogen，BUN)含量和丙氨酸氨基转移酶(alanine aminotransferase，ALT)活性，计算低密度脂蛋白胆固醇(low density lipoprotein-cholesterol，LDL-C)含量及动脉粥样硬化指数(atherosclerosis index，AI)；采用激光多普勒技术测定大鼠尾部微循环血流量。结果 疏肝调脂方3个剂量均能改善高脂血症大鼠的一般体征，降低血清TC、TG、LDL-C含量及AI，升高尾部微循环血流量(P<0.05或<0.01)；1.32 g·kg^-1能降低血清ALT活性(P<0.05)；1.32，2.64g·kg^-1能降低血清CR含量(P<0.05)。结论 疏肝调脂方可改善高脂血症模型大鼠的血脂水平、肝肾功能及微循环血流量，提示其具有调节血脂、改善肝肾损伤及微循环障碍的作用。

Abstract:

OBJECTIVE To investigate the effects of Shugan Tiaozhi Fang on blood lipid levels, liver and kidney function and microcirculation in hyperlipidemia model rats. METHODS The hyperlipidemia rat model was established by high-sugar and fat diets and alcohol-drinking. Two weeks after modeling, rats were given Shugan Tiaozhi Fang(0.66, 1.32, 2.64 g·kg^-1) once a day, each group were given continuous administration for 6 weeks. The general signs of the rats were observe before and after administration. The content of total cholesterol(TC), triglyceride(TG), high density lipoprotein-cholesterol(HDL-C), creatinine(CR), blood urea nitrogen(BUN) and the active of ALT in serum were determined. The content of low density lipoprotein-cholesterol(LDL-C) and atherosclerosis index(AI) were calculated. Microcirculation blood flow was detected by laser Doppler technology. RESULTS Shugan Tiaozhi Fang (0.66, 1.32, 2.64 g·kg^-1) improved the general signs of hyperlipidemia rats, reduced the content of TC, TG, LDL-C and AI(P<0.05 or 0.01), and increased the tail microcirculation blood flow(P<0.01). Shugan Tiaozhi Fang 1.32 g·kg^-1 dose reduced the active of ALT(P<0.05), and 1.32, 2.64g·kg-1 reduced the content of CR(P<0.05). CONCLUSION Shugan Tiaozhi Fang could ameliorate the serum lipid levels, liver and kidney function and microcirculation disorder of rats with hyperlipidemia, suggesting that it is capable of regulating blood lipids, ameliorate the liver and kidney damage and microcirculation microcirculation disorder.