丹参多酚酸盐对胶质瘤U251细胞增殖及凋亡的影响

Effect of Depsides Salts from Salvia Miltiorrhiza on Proliferation and Apoptosis of Glioma U251 Cells

关键词:  丹参多酚酸盐  胶质瘤  增殖  凋亡  自噬

Key words:  depsides salts from Salvia miltiorrhiza  glioma  proliferation  apoptosis  autophagy

摘要:

目的 研究丹参多酚酸盐对胶质瘤U251细胞增殖及凋亡的影响，并探讨其发生机制。方法 应用CCK-8试剂盒检测丹参多酚酸盐对U251细胞增殖的影响，流式细胞仪观察丹参多酚酸盐对U251细胞周期及细胞凋亡的影响，qRT-PCR检测自噬基因Beclin-1的表达。结果 丹参多酚酸盐可明显抑制胶质瘤U251细胞增殖，使细胞阻滞在G₀/G₁期，促进细胞凋亡，上调自噬基因Beclin-1 mRNA的表达。结论 丹参多酚酸盐可抑制胶质瘤U251细胞生长，诱导细胞发生凋亡，促凋亡效应可能与自噬基因Beclin-1 mRNA表达增多有关。

Abstract:

OBJECTIVE To investigate the effect of depsides salts from Salvia miltiorrhiza on proliferation and apoptosis of glioma U251 cells, and explore the possible mechanism. METHODS The effect of depsides salts from salvia miltiorrhiza on proliferation of U251 cells was assessed using the CCK-8 assay. Cell cycle analysis was done by flow cytometry. AnnexinV-FITC Apoptosis Detection Kit was used to detect apoptosis of cells．Real-time PCR(qRT-PCR) was employed to analyze expression of autophagy-related gene Beclin-1. RESULTS The growth inhibition of U251 cells by depsides salts from Salvia miltiorrhiza was in a dose-dependent manner. After treatment with 0.2 mg·mL^-1, cell cycle was arrested at G₀/G₁ phase, depsides salts from salvia miltiorrhiza also significantly enhanced apoptosis of U251 cells with up-regulated pro-autophagic gene Beclin-1. CONCLUSION Depsides salts from Salvia miltiorrhiza can inhibit the growth of glioma U251 cells, induce cell apoptosis, which may be related to raise expression of Beclin-1mRNA.