HPLC同时测定银菊浸膏中绿原酸、木犀草苷、3, 5-O-二咖啡酰基奎宁酸含量

Simultaneous Determination of Chlorogenic Acid, Mignonette Glycosides and 3, 5-O-dicaffeoylquinic Acid of Yinju Extract by HPLC

关键词:  菊花  金银花  绿原酸  木犀草苷  3, 5-O-二咖啡酰基奎宁酸  高效液相色谱法

Key words:  Chrysanthemi Flos  Lonicerae Japonicae Flos  chlorogenic acid  mignonette glycosides  3,5-O-dicaffeoylquinic acid  HPLC

摘要:

目的 建立同时测定银菊浸膏中绿原酸、木犀草苷、3, 5-O-二咖啡酰基奎宁酸含量的高效液相色谱法，优选最佳提取溶剂。方法 色谱条件：Ultimate PLUS C₁₈色谱柱(4.6 mm×250 mm，5 μm)，以乙腈(A)-0.1%磷酸水溶液(B)为流动相梯度洗脱，流速1.0 mL·min^-1，柱温40 ℃，波长348 nm，比较不同提取溶剂对浸膏中3种成分的影响。结果 绿原酸、木犀草苷、3, 5-O-二咖啡酰基奎宁酸分别在0.077~1.920，0.082~3.288，0.141~5.624 μg内线性关系良好，平均加样回收率为100.8%(RSD=2.3%)，101.5%(RSD=0.7%)，97.6%(RSD=0.6%)；银菊浸膏乙醇提取显著优于水提取，且40%乙醇提取最佳。结论 该法简便、准确，可用于银菊浸膏提取工艺优选与质量控制。

Abstract:

OBJECTIVE To establish an HPLC method for the simultaneous determination the contents of chlorogenic acid, mignonette glycosides and 3, 5-O-dicaffeoylquinic acid in Yinju extract and select the best extraction solvent. METHODS Ultimate PLUS C₁₈ column was applied(4.6 mm×250 mm, 5 μm) with acetonitrile(A) and 0.1% phosphoric acid(B) as mobile phase in gradient mode. The flow rate was 1.0 mL·min^-1 with column temperature of 40?℃. The detection wavelength was set at 348 nm. The contents of 3 components in Yinju extract extracted by different extraction solvents were compared. RESULTS The linear range of chlorogenic acid, mignonette glycosides and 3, 5-O-dicaffeoylquinic acid were 0.077-1.920, 0.082-3.288, 0.141-5.624 μg and the average recoveries were 100.8%(RSD=2.3%), 101.5% (RSD=0.7%), 97.6%(RSD=0.6%). Ethanol was better than water to extract Yinju extract and 40% ethanol was the best extraction solvent. CONCLUSION This method is simple, accurate and capable for the optimization of extract process and quality control of Yinju extract.