| 引用本文: | 罗兰,林久茂,魏丽慧,李琼瑜,薛曼华,彭军.余甘子醇提物抑制人胃癌株AGS细胞增殖和诱导细胞凋亡的作用[J].中国现代应用药学,2016,33(8):989-993. |
| LUO Lan,LIN Jiumao,WEI Lihui,LI Qiongyu,XUE Manhua,PENG Jun.Effects of Ethanol Extract of Phyllanthi Fructus on Proliferation and Apoptosis of Human Gastric Cancer AGS Cells[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(8):989-993. |
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| 摘要: |
| 目的 考察余甘子醇提物体外对人胃癌AGS细胞的生长抑制和诱导凋亡的作用。方法 采用MTT法、细胞集落实验、Hoechst染色分析、Annexin-V-FITC/PI等方法对经过余甘子醇提物体外作用的AGS细胞进行观察和检测。结果 AGS细胞经0.05，0.1，0.15 mg·mL-1余甘子醇提物作用后，各组细胞形态均发生不同程度的变化，并呈现浓度-时间依赖性；细胞集落实验表明余甘子醇提物抑制肿瘤细胞的集落形成率；Hoechst染色分析、Annexin V/PI实验表明余甘子醇提物能够促进肿瘤细胞的凋亡。结论 余甘子醇提物在体外对人胃癌AGS细胞具有良好的抑制生长和诱导凋亡的作用。 |
| 关键词: 余甘子 人胃癌AGS 细胞 抑制生长 诱导凋亡 |
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| 基金项目:福建省卫计委青年科研课题(2015-1-92) |
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| Effects of Ethanol Extract of Phyllanthi Fructus on Proliferation and Apoptosis of Human Gastric Cancer AGS Cells |
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LUO Lan1, LIN Jiumao2, WEI Lihui2, LI Qiongyu2, XUE Manhua1, PENG Jun2
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1.School of Pharmacy, Fujian Health College, Fuzhou 350101, China;2.Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
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| Abstract: |
| OBJECTIVE To investigate the effects of ethanol extract of Phyllanthi Fructus on proliferation and apoptosis of human gastric cancer AGS cells in vitro. METHODS After the action of alcohol extract of Phyllanthi Fructus in vitro, the AGS cells were observed and detectd through MTT method, Cell colony experiment, Hoechst staining, and Annexin V/PI. RESULTS After the treatment with 0.05, 0.10, 0.15 mg·mL-1 of alcohol extract, the morphology of AGS cells in each group changed different degrees and showed concentration-time dependence; Cell colony experiments showed that the ethanol extract of Phyllanthi Fructus can inhibit tumor cell colony formation rate; Hoechst staining analysis, Annexin-V-FITC/PI experiments showed that the ethanol extract of phyllanthi fructus can promote tumor cell apoptosis. CONCLUSION The alcohol extract of Phyllanthi Fructus in vitro has good inhibition activity and apoptosis induce on human gastric cancer AGS cells. |
| Key words: Phyllanthi Fructus human gastric cancer cell AGS growth inhibition induction of apoptosis |
