乌药醚内酯对肝损伤模型大鼠的保护作用

Hepatoprotective Effects of the Linderane on Liver Injury Model of Rats

关键词:  乌药醚内酯  超氧化物歧化酶  谷胱甘肽过氧化酶  丙二醛  谷氨酸转氨酶  天冬氨酸转氨酶

Key words:  linderane  SOD  GSH-Px  MDA  ALT  AST

摘要:

目的 考察不同剂量乌药醚内酯对大鼠肝损伤模型的保护作用。方法 60只健康SD大鼠，♂，随机分为空白对照组、模型对照组、乌药醚内酯低、中、高剂量组及联苯双酯组（阳性对照组），除空白对照组外，其余5组采用CCl₄腹腔注射建立肝损伤大鼠模型，造模期间各组给予相应的药物或等体积的水，造模6周后处死大鼠取血清，测定血清ALT、AST、SOD、GSH-Px活性及MDA含量，取肝脏组织染色切片观察病理变化。结果 与模型对照组对比，乌药醚内酯能剂量依赖性降低模型大鼠血清ALT、AST活性；高剂量组还能显著升高模型大鼠血清SOD、GSH-Px活性，降低MDA含量，显著降低肝脏组织的病理变化评分。结论 乌药醚内酯可能通过提高抗氧化能力抑制CCl₄诱导大鼠肝损伤。

Abstract:

OBJECTIVE To investigate the hepatoprotective effects of different doses of Linderane on liver injury using rat model. METHODS Sixty healthy male SD rats were randomly divided into 6 groups, which were control group, model group, linderane low-dose, medium-dose, high-dose group and positive control group. In addition to the control group, the other 5 groups were established liver injury model by carbon tetrachloride intraperitoneal injection. Each group was given either corresponding drug or the same volume of water during modeling. All the rats were killed after 6 weeks, and then the activity of ALT, AST, SOD, GSH-Px and the contents of MDA in serum were measured. The liver tissue sections were stained in order to observe their pathological changes. RESULTS Compared with the model group, linderane could decrease the activity of serum ALT and AST in model rats in a dose-dependent manner. The high-dose group could significantly increase the activity of serum SOD and GSH-Px, decrease serum MDA content, and significantly reduce the score of pathological changes of liver tissue in model rats. CONCLUSION The linderane could improve the antioxidant capacity against the liver injury induced by CCl₄ in rats.