| 引用本文: | 陈丹阳,林忠,潘牡丹,孙渊,金慧,洪盾,朱敏,谢群莉,陈利华,陈海啸.淫羊藿苷基于雌激素受体对糖皮质激素诱导的MC3T3-E1成骨抑制的影响[J].中国现代应用药学,2016,33(8):971-977. |
| CHEN Danyang,LIN Zhong,PAN Mudan,SUN Yuan,JIN Hui,HONG Dun,ZHU Min,XIE Qunli,CHEN Lihua,CHEN Haixiao.Regulate Effect of Icariin on Osteogenesis Inhibition of MC3T3-E1 Induced by Glucocorticoid in An Estrogen Receptor Dependent Manner[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(8):971-977. |
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| 淫羊藿苷基于雌激素受体对糖皮质激素诱导的MC3T3-E1成骨抑制的影响 |
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陈丹阳1, 林忠2, 潘牡丹3, 孙渊2, 金慧2, 洪盾4, 朱敏5, 谢群莉2, 陈利华5, 陈海啸4
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1.温州医科大学附属台州医院康复医学科，浙江 台州 317000;2.温州医科大学附属台州医院临床药学实验中心，浙江 台州 317000;3.临海市第一人民医院肿瘤外科，浙江 台州 317000;4.温州医科大学附属台州医院骨科，浙江 台州 317000;5.温州医科大学附属台州医院公共实验平台，浙江 台州 317000
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| 摘要: |
| 目的 研究淫羊藿苷(icariin，ICR)对雌性激素受体(estrogen receptor，ER)与地塞米松(dexamethasone，DEX)诱导的MC3T3-E1成骨抑制效应的影响。方法 分别以DEX 10-5 mol·L-1、ICR 10-6 mol·L-1、雌激素(E2)10-8 mol·L-1及ICI182780(IN)10-5 mol·L-1干预MC3T3-E1成熟分化过程，并通过real-time RT-PCR、Westen blot、MTT和茜素红染色法分别测定对应组各指标变化情况。结果 ICR和E2一样能够明显提高成骨细胞ALP、OPG、OC和Runx2的表达，并能够显著降低RANKL和Dickkopf的表达，对应的OPG和RANKL蛋白的表达量与mRNA的表达量相一致。ALP活性、细胞增殖能力以及Ga2+结节数量与对照组相比也有明显提高。同时ICR和E2都能够有效回复DEX诱导的成骨抑制效应，这种调节效应能够被IN有效的阻断。结论 ICR具有促成骨细胞增殖分化和抑制破骨细胞激活效应，并能够有效的缓解DEX诱导的成骨抑制效应；其促成骨效应具ER依赖性。 |
| 关键词: 淫羊藿苷 糖皮质激素 MC3T3-E1 雌激素受体 |
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| 基金项目:国家自然青年基金(31501147)；浙江省医药卫生科技计划项目(2015KYB436)；台州市科技基金(14SF03)；台州恩泽医疗中心科研基金(12EZD31)；浙江省台州恩泽医疗中心(集团)青蓝人才培养工程 |
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| Regulate Effect of Icariin on Osteogenesis Inhibition of MC3T3-E1 Induced by Glucocorticoid in An Estrogen Receptor Dependent Manner |
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CHEN Danyang1, LIN Zhong2, PAN Mudan3, SUN Yuan2, JIN Hui2, HONG Dun4, ZHU Min5, XIE Qunli2, CHEN Lihua5, CHEN Haixiao4
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1.Affiliated TaiZhou Hospital of Wenzhou Medical College, Department of Rehabilitation Medicine, Taizhou 317000, China;2.Affiliated TaiZhou Hospital of Wenzhou Medical College, Clinical Pharmacy Experimental Center, Taizhou 317000, China;3.The First People Hospital of Lin Hai, Oncological Surgery, Taizhou 317000, China;4.Affiliated TaiZhou Hospital of Wenzhou Medical College, Department of Orthopaedics, Taizhou 317000, China;5.Affiliated TaiZhou Hospital of Wenzhou Medical College, Public Experimental Platform, Taizhou 317000, China
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| Abstract: |
| OBJECTIVE To study the effect of icariin (ICR) on dexamethasone(DEX)-induced osteogenesis inhibition by activating the estrogen receptor (ER) in MC3T3-E1. METHODS The mature differentiation process of MC3T3-E1 was respectively interfered by adding DEX 10-5 mol·L-1, ICR 10-6 mol·L-1, estrogen(E2) 10-8 mol·L-1 and ICI182780(IN) 10-5 mol·L-1, and the real-time RT-PCR, Westen blot, MTT and alizarin red staining were applied for detecting the corresponding change of the indexes. RESULTS ICR could obviously increase the expression of ALP, OPG, OC and Runx2, and significantly reduce the expression of RANKL and Dickkopf in osteoblast as E2. Corresponding protein lever of OPG and RANKL were consistent with the mRNA expression. ALP activity, cell proliferation capacity and Ga2+ nodule number increased significantly compared with control group. Meanwhile ICR and E2 were able to effectively revert DEX-induced osteogenesis inhibition, and the regulating effect could be effectively blocked by IN. CONCLUSION ICR can improve osteoblast proliferation differentiation, inhibite osteoclast activation, and effectively relieve the DEX-induced inhibition effect with ER dependent. |
| Key words: icariin glucocorticoid MC3T3-E1 estrogen receptor |
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