中空纤维离心超滤法测定人血浆中双氯西林的游离浓度

Determination of Unbound Dicloxacillin in Human Plasma by Hollow Fiber Centrifugal Ultrafiltration

关键词:  中空纤维离心超滤  高效液相色谱法  双氯西林  游离血药浓度

Key words:  hollow fiber centrifugal ultrafiltration (HFCF-UF)  HPLC  dicloxacillin  unbound concentration

摘要:

目的 采用新型中空纤维离心超滤联合HPLC测定人血浆中双氯西林的游离浓度。方法 采用中空纤维离心超滤法对血浆样品进行预处理。采用Kromasil C₁₈色谱柱进行HPLC测定，流动相为乙腈-25 mmol·L^-1磷酸盐缓冲溶液（pH4.5）=40：60，流速1.0 mL·min^-1，检测波长225 nm，柱温20℃。结果 双氯西林在64.0~4 096.0 ng·mL^-1内线性关系良好（r=0.999 9），定量限为32.0 ng·mL^-1，回收率为98.8%~100.3%，RSD<1.3%。结论 本方法简便、快速、专属性强、灵敏度高，为双氯西林的游离浓度测定提供了一种新的、可靠的分析手段。

Abstract:

OBJECTIVE To establish a novel method combining hollow fiber centrifugal ultrafiltration and HPLC for determination of unbound dicloxacillin in human plasma. METHODS Hollow fiber centrifugal ultrafiltration was employed for the pretreatment of plasma samples. The Kromasil C₁₈ column was used for HPLC analysis. The chromatographic conditions were as follows, mobile phase was acetonitrile-25 mmol·L^-1 phosphate solution (pH 4.5)=40:60; flow arte was 1.0 mL·min^-1; detection wavelength was 225 nm; column temperature was 20℃. RESULTS Unbound dicloxacillin showed good linear relationship in the range of 64.0-4096.0 ng·mL^-1(r=0.999 9), and the limit of quantification was 32.0 ng·mL^-1. The recovery were 98.8%-100.3% with RSD<1.3%. CONCLUSION The method is simple, rapid, specific and sensitive. It provides a new reliable means for determination of unbound dicloxacillin.