纳米山药多糖对4种肿瘤细胞的作用

Effect of Nano Chinese Yam Polysaccharide on 4 Kinds of Tumor Cells

关键词:  抗肿瘤  纳米山药多糖  CCK-8  Western blotting  细胞划痕实验

Key words:  anti-tumor  nano chinese yam polysaccharide  CCK-8  Western blotting  cell scratch experiment

摘要:

目的 研究纳米山药多糖的抗肿瘤活性，并对其作用机制进行探究。方法 细胞增殖/毒性检测试剂盒(CCK-8试剂盒)测定各给药组对肿瘤细胞抑制率的影响；Western blotting检测不同给药组给药48 h后对4种肿瘤细胞凋亡相关蛋白caspase-3、caspase-8表达情况的影响；细胞划痕实验检测各给药组对肿瘤细胞迁移能力的影响。结果 CCK-8实验表明，与对照组相比，纳米山药多糖高剂量组(80 ng·mL^-1)、氟尿嘧啶组(80 ng·mL^-1)能够明显抑制肿瘤细胞增殖(P<0.05)；Western blotting实验显示纳米山药多糖高剂量组(80 ng·mL^-1)与氟尿嘧啶组(80 ng·mL^-1)肿瘤细胞凋亡蛋白caspase-3、caspase-8条带灰度降低，蛋白含量表达与对照组比较明显减少(P<0.05)；细胞划痕实验表明纳米山药多糖作用于4种肿瘤细胞48 h后，细胞愈合率均有不同程度的下降(P<0.05)。结论 纳米山药多糖剂量的升高，促进肿瘤细胞凋亡蛋白caspase-3和caspase-8蛋白酶原的活化，酶原降解增多，抑制肿瘤细胞生长，促进肿瘤细胞凋亡，降低细胞划痕愈合率，具有抗肿瘤活性。

Abstract:

OBJECTIVE To study anti-tumor activity of nano chinese yam polysaccharide and to explore the mechanism of its action. METHODS Cell proliferation/toxicity detection kit(CCK-8 kit) was used to determine inhibition rate to tumor cells of each drug administration group; Western blotting was used to test the effect of each drug administration group on the expression of tumor cell apoptosis related protein caspase-3, caspase-8 after 48 h. Effect of each drug administration group on the ability of tumor cell migration was tested by tumor cell scratch experiment. RESULTS CCK-8 experiment showed that compared with normal control group, high dose group of nano chinese yam polysaccharide(80 ng·mL^-1) and fluorouracil group(80 ng·mL^-1) could obviously inhibit tumor cell proliferation(P<0.05); Western blotting experiment showed that the tumor cell apoptosis protein caspase-3, caspase-8 of nano chinese yam polysaccharide group(80 ng·mL^-1) and fluorouracil group(80 ng·mL^-1) had lower band grayscale, protein expression were significantly increased compared with the normal group (P<0.05); Cell scratch experiment showed that healing rate of 4 kinds of tumor cells treated with nano chinese yam polysaccharide all had varying degrees of decline(P<0.05) after 48 h. CONCLUSION The increase of the dosage of nano chinese yam polysaccharide can promote the original tumor cell apoptosis caspase-3 and caspase-8 protease activation, increase the degradation of zymogen, inhibit the growth of tumor cells, promote tumor cell apoptosis and reduce cell scratch healing rate, nano chinese yam polysaccharide has anti-tumor activity.