| 引用本文: | 张朝阳,闫中天,杨锐,孙会敏.HPLC测定卡波姆共聚物中残留的丙烯酸含量[J].中国现代应用药学,2016,33(8):982-984. |
| ZHANG Zhaoyang,YAN Zhongtian,YANG Rui,SUN Huimin.Determination of Acrylic Acid in Carbomer Copolymer by HPLC[J].Chin J Mod Appl Pharm(中国现代应用药学),2016,33(8):982-984. |
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| 摘要: |
| 目的 采用HPLC测定卡波姆共聚物中残留的丙烯酸含量。方法 使用Sepax GP-C18柱(4.6 mm×250 mm，5 μm)分离，流动相为磷酸二氢钾-甲醇(8∶2)，流速为1.0 mL·min-1，检测波长200 nm，柱温为30 ℃。结果 卡波姆共聚物中的残留丙烯酸可达到满意的分离，丙烯酸浓度在0.327 5~32.75 μg·mL-1内有良好的线性关系(r=0.999 6)，方法平均回收率为93.02%。结论 本方法准确，可靠，重现性好，可作为卡波姆共聚物中丙烯酸残留的控制方法。 |
| 关键词: 卡波姆共聚物 高效液相色谱法 丙烯酸 含量测定 |
| DOI： |
| 分类号: |
| 基金项目:“重大新药创制”国家科技重大专项(2015ZX09303001) |
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| Determination of Acrylic Acid in Carbomer Copolymer by HPLC |
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ZHANG Zhaoyang, YAN Zhongtian, YANG Rui, SUN Huimin
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National Institutes for Food and Drug Control, Beijing 100050, China
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| Abstract: |
| OBJECTIVE To establish an HPLC method for the determination of acrylic acid in carbomer copolymer. METHODS The samples were separated on Sepax GP-C18 column (4.6 mm×250 mm, 5 μm) eluted with potassium dihydrogen phosphate-methanol(80∶20) as mobile phase. The isocratic elution was used at a flow rate of 1.0 mL·min-1, the detection wavelength was 200 nm, the column temperature was 30 ℃. RESULTS The calibration curve was linear in the range of 0.327 5-32.75 μg·mL-1 for acrylic acid with r=0.999 6, the average recovery was 93.02%. CONCLUSION The method is accurate and reliable, and can be applied for the quality control of acrylic acid in carbomer copolymer. |
| Key words: carbomer copolymer HPLC acrylic acid content determination |
