穿心莲提取物对口腔溃疡豚鼠细胞因子表达的影响

Effect of Andrographis Paniculata Extract on the Expression of Cytokines in Oral Ulcer Guinea Pigs

关键词:  穿心莲提取物  口腔溃疡豚鼠  TNF-α  IL-6  VEGF

Key words:  Andrographis Paniculata extract  oral ulcer guinea pig  TNF-α  IL-6  VEGF

摘要:

目的 探讨穿心莲提取物(andrographis paniculata extract，APE)对口腔溃疡豚鼠细胞因子表达的影响。方法 用90%苯酚溶液灼烧豚鼠左侧面颊，造成口腔溃疡模型，观察APE对口腔溃疡豚鼠溃疡面积、溃疡程度及组织病理变化，给药5 d后测定血清肿瘤坏死因子(TNF-α)、白介素-6(IL-6)、血管内皮生长因子(VEGF)的表达。结果 用苯酚溶液制作豚鼠口腔溃疡模型成功，与模型组比较，高剂量APE外用组和桂林西瓜霜组均可显著减小溃疡面积(P<0.01)，明显改善口腔溃疡程度和溃疡的病理变化，显著降低血清TNF-α水平(P<0.01)和IL-6水平(P<0.01)，VEGF表达有显著差异(P<0.01)，且2组间无显著差异。结论 APE对口腔溃疡豚鼠有治疗作用。

Abstract:

OBJECTIVE To discuss the effect of Andrographis paniculata extract (APE) on the expression of cytokines in oral ulcer guinea pigs. METHODS Oral ulcer model of guinea pig was induced by burning the left cheek with 90% phenol solution. Effects of APE on ulcer area, ulcer degree and ulcer tissue pathological changes of oral ulcer guinea pigs were observed. Expressions of tumor necrosis factor-α(TNF-α), interleukin 6 (IL-6) and vascular endothelial growth factor (VEGF) in serum were determined after administered continuously for 5 d. RESULTS Oral ulcer model of guinea pigs was induced by phenol solution successfully. Compared with the model group, the high dosage of APE external group and the Guilin Watermelon Frost group could significantly decrease ulcer area(P<0.01), significantly improve the degree of oral ulcer and the pathological changes of ulcer, significantly reduce levels of TNF-α and IL-6(P<0.01) and significantly express difference of VEGF (P<0.01), there was no significant difference between the two groups. CONCLUSION APE has therapeutic effect on oral ulcer guinea pigs.