鹅不食草油鼻用微乳温敏凝胶释药系统鼻黏膜刺激性及主要脏器影响研究

Study on the Nasal Irritation and Main Organic Effect of the Drug Delivery System of Centipeda Minima Oil Microemulsion-based Thermosensitive Hydrogel

关键词:  鹅不食草油  微乳  温敏凝胶  鼻黏膜  刺激性

Key words:  Centipeda minima oil  microemulsion-based  temperarure-sensitive gel  nasal  irritation

摘要:

目的 考察鹅不食草油鼻用微乳温敏凝胶剂对大鼠鼻腔黏膜的刺激作用及主要脏器的形态学变化。方法 大鼠随机分成空白对照组、空白微乳温敏凝胶组及鹅不食草油鼻用微乳温敏凝胶组，考察大鼠鼻腔给药后鼻黏膜及主要脏器形态学结构变化，评价鹅不食草油鼻用微乳温敏凝胶的初步安全性。结果 大鼠鼻黏膜组织切片显示各组的鼻中隔软骨上均有完整纤毛和正常的黏膜细胞存在，未见明显的血管充血现象，也均未见明显的黏膜、组织细胞坏死、脱落及出血现象；主要脏器组织切片图结构显示大鼠鼻腔给药后与空白对照组及空白微乳温敏凝胶组一样均未见组织细胞结构病变、水肿、坏死等变化。结论 鹅不食草油鼻用微乳温敏凝胶释药系统对大鼠鼻黏膜无明显刺激性，对主要脏器未见明显损伤，提示其应用具有用药的安全性。

Abstract:

OBJECTIVE To investigate the irriration and organic morphological variation of Centipeda minima oil microemulsion-based thermosensitive hydrogel in rats. METHODS The rats were divided into 3 groups, including the normal control group, the blank microemulsion-based thermosensitive hydrogel group and the Centipeda minima oil microemulsion-based thermosensitive hydrogel group, investigating the morphological changes of main organs of rats after nasal administration, to evaluate the preliminary security of Centipeda minima oil microemulsion-based thermosensitive hydrogel. RESULTS Rat nasal mucosa tissue sevtions showed that there was completed cilia and normal mucous cells in the nasal septum cartilage after nasal administration of saline solution, blank gel and Centipeda minima oil microemulsion-based thermosensitive hydrogel, without obvious vascular congestion, necrosis, shedding, bleeding of mucosa and tissue. The histological sections of main organs of the rats after nasal administration of Centipeda minima oil microemulsion-based thermosensitive hydrogel revealed that there was no cell structural changes, such as the normal group and the blank gel group. CONCLUSION The Centipeda minima oil microemulsion-based thermosensitive hydrogel is approved to have no obvious irritation to the nasal mucosa and no obvious damage on main organs of rats, and it is suggested that the application of Centipeda minima oil microemulsion-based thermosensitive hydrogel is safe.