大黄素对脓毒症大鼠血糖和胰岛素抵抗的影响

Effect of Emodin on Hyperglycemia and Insulin Resistance of Sepsis Rats

关键词:  大黄素  脓毒症  高血糖  胰岛素抵抗

Key words:  emodin  sepsis  hyperglycemia  insulin resistance

摘要:

目的 研究大黄素对脓毒症大鼠高血糖及胰岛素抵抗的影响。方法 60只SD大鼠随机分为假手术组、模型组、大黄素组、二甲双胍阳性对照组。手术前1 h，大黄素组灌胃给予100 mg·kg^-1的CMC-Na混悬液，二甲双胍阳性对照组灌胃给予150 mg·kg^-1的CMC-Na混悬液，按照盲肠结扎穿刺法建立脓毒症模型。分别测定术前及术后1，2，4，6，12，24 h的血糖值，及术后2 h的胰岛素水平，计算胰岛素抵抗指数，并于术后24 h腹主动脉取血，酶联免疫吸附法检测IL-6、TNF-α、CRP水平。结果 大黄素能改善脓毒症大鼠的胰岛素抵抗，抑制脓毒症大鼠感染后血糖的升高，并能明显降低炎症因子IL-6、TNF-α、CRP的水平。结论 大黄素能降低脓毒症大鼠的高血糖，改善其胰岛素抵抗，其作用机制可能与抑制炎症细胞因子分泌有关。

Abstract:

OBJECTIVE To investigate the effects of emodin on hyperglycemia and insulin resistance of the sepsis rats. METHODS Sixty SD rats were randomly divided into sham operation group, model group, emodin group and metformin positive control group. At 1 h before operation, emodin group rats were given 100 mg·kg^-1 CMC-Na suspension by gavage and 150 mg·kg^-1 CMC-Na for metformin positive control group, sepsis model were based on cecal ligation and puncture. The blood glucose in rats were measured at 0, 1, 2, 4, 6, 12, 24 h before and after operation, and at 2 h after operation, the insulin levels were measured, at 24 h after operation, IL-6, TNF-α, CRP level were measured by enzyme linked immunosorbent assay. RESULTS Emodin could improve the insulin resistance, lower the high blood glucose of infected sepsis rats and significantly reduce the level of IL-6, TNF-α and CRP. CONCLUSION Emodin can reduce hyperglycemia of sepsis rats, and improve the insulin resistance. Its protective mechanism may be associated with inhibiting the inflammatory cytokine secretion.