原子荧光法测定苄达赖氨酸滴眼剂中抑菌剂硫柳汞的含量

Determination of the Content of the Merthiolate in the Bendazac Lysine Eye Drops by Atomic Fluorescence Spectrometry

关键词:  原子荧光分光光谱法  苄达赖氨酸滴眼剂  抑菌剂  硫柳汞

Key words:  atomic fluorescence spectrometry  bendazac lysine eye drops  bacteriostatic agent  merthiolate

摘要:

目的 建立苄达赖氨酸滴眼剂中抑菌剂硫柳汞的原子荧光光谱含量测定方法。方法 采用微波消解系统消解样品后，用双道原子荧光光谱仪测定苄达赖氨酸滴眼剂中硫柳汞的含量。结果 3家企业样品的平均含量分别为0.001%，0.030%和0.001%，方法检出限为0.013 4 μg·L^-1，线性范围为0.4~2.0 μg·L^-1，线性回归方程相关系数为0.999 3，平均回收率为99.2%。结论 采用本法测定滴眼剂中硫柳汞的含量灵敏准确，专属性强，干扰少，对于控制滴眼剂中硫柳汞含量有实际意义。

Abstract:

OBJECTIVE To establish an atomic fluorescence spectrometry method for the determination of the content of the merthiolate in the bendazac lysine eye drops. METHODS Determination of the content of the merthiolate in the bendazac lysine eye drops by dual channel atomic fluorescence spectrometry after digestion of samples with microwave digestion system. RESULTS The average content of the sample of 3 enterprises was 0.001%, 0.030%, and 0.001%, respectively, the detection limit was 0.013 4 μg·L^-1, the linear range was 0.4-2.0 μg·L^-1, the correlation coefficient was 0.999 3, and the average recovery was 99.2%. CONCLUSION Using this method to determine the content of the merthiolate in the bendazac lysine eye drops is sensitive and accurate, with strong specificity and little interference. It is of practical significance for the control of the content of merthiolate in the eye drops.