沙参麦冬汤中3种有效成分血药浓度的UPLC-MS/MS测定及药动学研究

Determination of Plasma Consentration of 3 Active Components in Shashen Maidong Decoction by UPLC-MS/MS and Their Pharmacokinetic Study

关键词:  UPLC-MS/MS  沙参麦冬汤  甘草苷  花椒毒酚  麦冬黄烷酮A  药动学

Key words:  UPLC-MS/MS  Shashen Maidong decoction  liquiritin  xanthotoxin  methylophiopogonanone A  parmacokinetic

摘要:

目的 建立大鼠血浆中甘草苷、花椒毒酚和甲基麦冬黄烷酮A的UPLC-MS/MS测定方法，并探讨大鼠灌胃沙参麦冬汤后其在大鼠体内的药动学过程。方法 以流动相乙腈-0.1%甲酸水溶液，梯度洗脱，流速0.3 mL·min^-1；采用ESI源，正负离子同时检测模式扫描，多反应监测模式（MRM）检测各成分血药浓度，并用DAS 3.0软件计算药动学参数。结果 甘草苷、花椒毒酚和麦冬黄烷酮A分别在4.92~315.00 ng·mL^-1、1.44~92.00 ng·mL^-1和0.35~22.00 ng·mL^-1线性关系良好，平均回收率均>76.5%，日内、日间RSD均<15%。大鼠灌胃沙参麦冬汤提取物后，甘草苷、花椒毒酚和甲基麦冬黄烷酮A的AUC_0-t分别为（718.23±185.55）ng·h·mL^-1，（22.52±7.53）ng·h·mL^-1和（13.55±6.03）ng·h·mL^-1；t_1/2分别为（3.61±2.01）h，（6.93±7.78）h和（3.51±1.92） h。结论 本法方便、快捷，可用于甘草苷、花椒毒素和甲基麦冬黄烷酮A的体内定量分析。

Abstract:

OBJECTIVE To establish an UPLC-MS/MS method for the determination of 3 components as liquiritin, xanthotoxin and methylophiopogonanone A of Shashen Maidong decoction in rat plasma, and to study the pharmacokinetics of them. METHODS The choromatography was eluted with mobile phase consisted of acetonitrile and 0.1% formic acid solution at the flow rate of 0.3 mL·min^-1 by gradient elution. Multiple-reaction monitoring (MRM) scanning mode was employed for quantification with switching electrospray ion (ESI) source polarity in positive/negative mode. The pharmacokinetic parameters were calculated by using DAS 3.0 software. RESULTS The linear ranges of liquiritin, xanthotoxin and methylophiopogonanone A were 4.92-315.00 ng·mL^-1, 1.44-92.00 ng·mL^-1 and 0.35-22.00 ng·mL^-1, respectively. RSDs of inta-day and inter-day were all lower than 15%, and the average recoveries were more than 76.5%. The pharmacokinetic parameters of liquiritin, xanthotoxin and methylophiopogonanone A were as follows:AUC_0-t were (718.23±185.55) ng·h·mL^-1, (22.52±7.53) ng·h·mL^-1, and (13.55±6.03) ng·h·mL^-1; t_1/2 were (3.61±2.01)h, (6.93±7.78)h and (3.51±1.92)h. CONCLUSION The method is convenient, sensitive and specific, and can be used for analysis of liquiritin, xanthotoxin and methylophiopogonanone A in vivo.