基于多指标综合评分法优化参黄散提取工艺

Optimization of Extraction Process for Shenhuang Recipe Based on Multi-index Comprehensive Evaluation Method

关键词:  参黄散  正交设计  层次分析法  提取工艺

Key words:  Shenhuang recipe  orthogonal test  analytic hierarchy process  extraction technology

摘要:

目的 优选参黄散的提取工艺。方法 以层次分析法对大黄素、大黄酸、大黄酚、厚朴酚、丹参酮ⅡA的提取率、浸膏得率等6个指标进行权重分配计算综合评分，采用正交设计试验法考察乙醇浓度、药液比、提取次数对提取效果的影响，并对优化的提取工艺进行验证试验。结果 较优的提取工艺为采用6倍体积60%乙醇，80℃水浴加热回流提取3次，每次1 h。验证试验显示大黄素、大黄酸、大黄酚、厚朴酚、丹参酮ⅡA的提取率分别为70.48%，57.36%，83.87%，49.18%，83.87%；浸膏得率为37.27%。结论 优选的提取工艺简便、稳定、成本低，可用于参黄散的提取，有助于促进参黄散复方制剂的研究。

Abstract:

OBJECTIVE To optimize the extraction technology of Shenhuang recipe. METHODS Comprehensive evaluation score by analytic hierarchy process as the 6 indexs, such as the free anthraquinones in Rhubarb, honokiol and tanshinone Ⅱ_A extraction rate, extract rate. The effects of ethanol concentration, drug-solvent ratio and extraction times on extraction were investigated by orthogonal test. The extraction technology was optimized and validation test was conducted. RESULTS The optimum extraction process was as follows, 6-fold 60% ethanol, 80℃ water bath heating reflux extracting 3 times, 1 h each time. The validation test showed that the average extraction rate of emodin, rhein, chrysophanol, honokiol and tanshinone Ⅱ_A were 70.48%, 57.36%, 83.87%, 49.18%, 83.87%, and the average extract rate was 37.27%. CONCLUSION The optimized extraction process is simple, stable and low cost, which can be used for the extraction of Shenhuang recipe, and it is helpful to promote the study of the compound preparation.