构树总黄酮对免疫抑制小鼠免疫功能的影响

Immunomodulatory Effects of Total Flavonoids of Broussonetia Papyrifera on Immunosuppressed Mouse

关键词:  构树总黄酮  免疫抑制  免疫调节

Key words:  total flavonoids of Broussonetia papyrifer  immunosuppression  immunomodulatory

摘要:

目的 探讨构树总黄酮（total flavonoids of Broussonetia papyrifera，TFBP）对环磷酰胺诱导的免疫抑制小鼠免疫功能的影响。方法 用环磷酰胺复制免疫抑制模型并随机分为模型组、构树总黄酮高、中、低剂量组（200，100，50 mg·kg^-1·d^-1），以正常小鼠为空白对照组。分别于饲喂构树总黄酮后第10天和第30天采样，检测白细胞含量、腹腔巨细胞吞噬功能、T、B淋巴细胞转化率、血清IgG含量及BSA抗体水平。结果 与模型组比较，饲喂TFBP的高、中、低剂量组小鼠的白细胞含量、腹腔巨噬细胞吞噬功能、T、B淋巴细胞转化率、BSA抗体水平和血清IgG均显著升高（P < 0.05或P < 0.01），随着TFBP剂量的增加，上述免疫指标与空白对照组相近。结论 构树总黄酮能显著改善环磷酰胺诱导的免疫抑制小鼠的免疫功能。

Abstract:

OBJECTIVE To investigate the immunomodulatory effects of the total flavonoids of broussonetia papyrifera(TFBP) on immunosuppressed mice induced by cyclophosphamide(CTX). METHODS SPF Kunming mice were randomly divided into control group, model group, and TFBP high, middle, low dosage groups(200, 100, 50 mg·kg^-1·d^-1). Except control group, the other groups were administered CTX by peritoneal injection to induce immunosuppression status. After 10 d and 30 d, the blood samples were collected to determine the number of white blood cells, phagocytosis of macrophage cells, serum IgG content, BSA antibody titers and proliferation of T and B lymphocytes. RESULTS The number of white blood cells, phagocytosis of macrophage cells, proliferation of T, B lymphocyte, BSA antibody levels and serum IgG content of the mice in TFBP groups were significantly increased(P < 0.01 or P < 0.05) in a dose-dependent manner than that of model group. Moreover, as the TFBP increased in the diets, the data above were closed to that of normal mice in control group. CONCLUSION The immune function of the immunosuppressed mice induced by CTX can be improved significanltly by TFBP administration.