黄连碱对慢性肾功能衰竭大鼠的治疗作用及其机制研究

Effect and Mechanism of Coptisine on the Rat Model of Chronic Renal Failure

关键词:  黄连碱  肾功能衰竭  TGF-β1  PAI-1

Key words:  coptisine  chronic renal failure  TGF-β₁  PAI-1

摘要:

目的 探讨黄连碱对慢性肾功能衰竭（chronic renal failure，CRF）大鼠的治疗作用及其机制。方法 60只健康SD大鼠，♂，随机分为空白组、模型组、尿毒清组、黄连碱200，100和50 mg·kg^-1剂量组，每组10只。除空白组外，各组大鼠灌胃腺嘌吟，连续28 d，制成CRF模型。造模同时，黄连碱200，100和50 mg·kg^-1剂量组每日分别灌胃200，100和50 mg·kg^-1黄连碱。治疗后，观察各组大鼠体质量的变化、检测24 h尿量及尿蛋白含量，Elisa法检测各组大鼠血清尿素氮（BUN）、肌酐（SCr）、TGF-β1及PAI-1的含量水平、Western blot法检测大鼠肾组织TGF-β1及PAI-1蛋白的表达。结果 与模型组比较，200，100和50 mg·kg^-1剂量的黄连碱干预后，大鼠体质量及24 h尿量显著增加（P<0.05），尿蛋白显著降低（P<0.05），且血清中BUN、SCr、TGF-β1及PAI-1含量和肾组织TGF-β1和PAI-1表达均显著降低（P <0.05）。结论 黄连碱对CRF大鼠的肾功有一定的改善作用，其机制与降低血清及肾组织中TGF-β1和PAI-1表达有关。

Abstract:

OBJECTIVE To explore the effect and mechanism of coptisine on the rat model of chronic renal failure(CRF). METHODS The 60 male SD rats were divided into 6 groups:the blank group, model group, positive control group and the 200,100 and 50 mg·kg^-1 dose of coptisine groups. Rats were gavaged adenine 28 d, except the blank group. The high, middle, low dose of coptisine group rats were gavaged 200, 100 and 50 mg·kg^-1 coptisine each day. After therapy, weight, urinary production and urinary protein in 24 h was detected, urea nitrogen(BUN), creatinine(SCr), TGF-β₁ and PAI-1 in serum was detected by Elisa; the expression of TGF-β₁ and PAI-1 in kidney tissue was detected by Western blot. RESULTS Compared with model group, in 200, 100 and 50 mg·kg^-1 dose of coptisine groups, weight and urinary production of 24 h were increased(P<0.05) and urinary protein in 24 h was decreased(P<0.05); BUN, SCr, TGF-β₁ and PAI-1 level in serum and the expression of TGF-β₁ and PAI-1 protein was decreased(P<0.05). CONCLUSION Coptisine has a certain effect on the renal funtion of CRF rats and its mechanism is related to the expression of TGF-β₁ and PAI-1 in srtum and renal tissue.