甲基-β-环糊精手性流动相添加剂法拆分射干中异构体和结构相近化合物

Separation of Isomer and Similar Structure Compounds in Belamcandae Rhizoma by Methyl-β-CD Chiral Mobile Phase Additive HPLC

关键词:  射干  手性流动相添加剂法  甲基-β-环糊精  同分异构体  高效液相色谱法

Key words:  Belamcandae Rhizoma  chiral mobile phase additive  methyl-β-cyclodextrin  isomers  HPLC

摘要:

目的 建立射干中异构体和结构相近化合物反相高效液相手性流动相拆分分析方法。方法 采用Kromasil C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相水相含0.5%甲基-β-环糊精、0.1%磷酸，有机相为乙腈，梯度洗脱，柱温30℃，检测波长为265 nm。结果 成功拆分鸢尾甲黄素A、B同分异构体以及与其结构相近的野鸢尾黄素，并且能同时测定射干中的8个成分。结论 该方法使射干达到基线分离，有效缩短分析时间、耐用性好、分析成本低。

Abstract:

OBJECTIVE To establish the separation method of isomer and similar compounds in Belamcandae Rhizoma with RP-HPLC. METHODS The separation was performed on Kromasil C₁₈ column (250 mm×4.6 mm, 5 μm) with mobile phase of aqueous phase with 0.5% methyl-β-cyclodextrin and 0.1% phosphate, organic phase for acetonitrile, gradient elution. The column temperature was 30℃, and the detection wavelength was 265 nm. RESULTS Successful split isomer of iristectorigenin A with iristectorigenin B, with similar structure of irigenin. And it was possible to measure 8 compounds in Belamcandae Rhizoma at the same time. CONCLUSION Belamcandae Rhizoma can be baseline-separated by this method which is simple and economic with good resolution.