HPLC测定肾炎宁片中大黄酸、大黄素、大黄酚及雷公藤甲素的含量

Determination of Arietic Acid, Emodin, Chrysophanol and Triptolide in Shenyanning Tablets by HPLC

关键词:  肾炎宁片  大黄酸  大黄素  大黄酚  雷公藤甲素  高效液相色谱法  含量测定

Key words:  Shenyanning tablets  parietic acid  emodin  chrysophanol  triptolide  HPLC  determination

摘要:

目的 建立HPLC测定肾炎宁片中大黄酸、大黄素、大黄酚及雷公藤甲素的含量。方法 大黄酸、大黄素、大黄酚的测定采用流动相为甲醇-0.1%磷酸（70∶30），检测波长为254 nm；雷公藤甲素采用流动相为乙腈-水（25∶75），检测波长为220 nm；两者均采用Lichrospher-C₁₈柱（4.6 mm×250 mm，5 mm），流速均为1.0 ml·min^-1。结果 大黄酸、大黄素、大黄酚以及雷公藤甲素分别在1.83~14.64 μg·ml^-1（r=0.999 9），2.895~23.16 μg·ml^-1（r=0.999 8），7.625~61.00 μg·ml^-1 （r=0.999 8）以及1.52~24.32 μg·ml^-1（r=0.999 6）内与峰面积呈良好的线性关系，大黄酸平均回收率为103.2%，RSD=1.73% （n=9）；大黄素平均回收率为98.6%，RSD=3.94%（n=9）；大黄酚平均回收率为98.3%，RSD=2.54%（n=9）；雷公藤甲素平均回收率为99.61%，RSD=2.83%（n=9）。结论 本方法准确、可靠、专属性强，可作为该制剂的定量控制方法。

Abstract:

OBJECTIVE To establish a quantitative method for parietic acid, emodin, chrysophanol and triptolide in shenyanning tablets by HPLC. Methods the mobile phase for determining parietic acid, emodin, chrysophanol was methanol-0.1% phosphoric acid(70∶30), the UV detection wavelength was 254 nm, while the mobile phase for triptolide was acetonitrile-water (25∶75), the UV detection wavelength was 220 nm. Lichrospher C₁₈(4.6 mm×250 mm, 5 mm) column was used for the determination, and the flow rate was 1.0 ml·min^-1.RESULTS The linear range of parietic acid, emodin, chrysophanol, and triptolide was 1.83-14.64 μg·ml^-1(r=0.999 9), 2.895-23.16 μg·ml^-1(r=0.999 8), 7.625-61.00 μg·ml^-1(r=0.999 8) and 1.52-24.32 μg·ml^-1 (r=0.999 6). The average recover was 103.2%, RSD=1.73%(n=9), 98.6%, RSD=3.94%(n=9), 98.3%, RSD=2.54%(n=9) and 99.61%, RSD=2.83%(n=9) for parietic acid, emodin, chrysophanol, and triptolide. CONCLUSION The method is accurate, reliable and apecific, can be used in the quality control of Shenyanning tablets.