血必净对脓毒症大鼠肾脏细胞凋亡及相关蛋白表达的影响

Effects of Xuebijing Injection on Tubular Apoptosis and Associated Protein Expression in Sepsis Induced Acute Kidney Injure Rats

关键词:  血必净  脓毒症  急性肾损伤  凋亡  Bcl-2/Bax

Key words:  Xuebijing  sepsis  acute kidney injury  apoptosis  Bcl-2/Bax

摘要:

目的 探讨早期应用血必净对脓毒症导致急性肾损伤后肾小管细胞凋亡的影响，及凋亡相关蛋白Bcl-2和Bax表达的变化。方法 54只SD大鼠，♂，随机分为假手术组、脓毒症组、血必净组，每组18只；各组再分为3个亚组，每组6只。根据实验要求分别以术后12，24，48 h作为时间观察点，在各时间点检测大鼠的血液生化指标，测定肾脏血流、并留取肾脏组织观察病理学变化，测定肾小管损伤评分，采用原位末端标记法测定肾小管凋亡细胞，并计算积分光密度值（IOD）。采用免疫印迹法测定Bcl-2、Bax的变化。结果 血必净组能明显改善肾功能，稳定血流动力学，减轻组织病理学变化，减少肾小管细胞凋亡，肾小管损伤评分和IOD值与脓毒症组相比有显著差异（P<0.01）。同时，血必净组能升高Bcl-2的表达，抑制Bax的表达，保持Bcl-2/Bax的平衡，与脓毒症组相比有显著差异（P<0.01）。结论 早期应用血必净可以减少肾小管细胞在脓毒症进程中的凋亡，改善脓毒症导致急性肾损伤的病理学改变，保持Bcl-2/Bax的平衡，减轻脓毒症导致的肾损伤。

Abstract:

OBJECTIVE To investigate the effect of early application of Xuebijing injection in sepsis induced acute kidney injure(SI-AKI) by observing tubular apoptosis and Bcl-2/Bax expression.METHODS Fifty-four male SD rats were randomly divided into 3 groups:sham group (sham), sepsis group and Xuebijing group, eighteen rats in each group. Each group were divided into 3 sub-groups, six rats in each group. After model was established, the phases of 12, 24, 48 h were respectively regarded as observation point, and blood biochemical parameters, renal blood flow, renal pathological changes and renal tubular injury scores were measured; renal tubular cell apoptosis was determined by TUNEL method and integrated optical density (IOD) was calculated. The expressions of Bcl-2 and Bax were detected by Western blot.RESULTS Xuebijing injection could significantly improve renal function, hemodynamics of kidney, pathological changes and reduce tubular cell apoptosis. Tubular damage scores and the IOD had a significant difference in Xuebijing group compared with CLP group(P<0.01). Meanwhile, Xuebijing injection could increase the expression of Bcl-2, and inhibit the Bax, keep the balance of Bcl-2/Bax ratio; there were significant differences(P<0.01) in Xuebijing group compared with sepsis group.CONCLUSION Early application of Xuebijing injecton may improve renal dysfunction and the pathological changes, reduce the tubular cell apoptosis, keep the balance of Bcl-2/Bax ratio in SI-AKI.