板蓝根水提物对糖尿病大鼠早期肝损伤的影响

Effect of Water Extract of Radix Isatidis on Early Liver Injury in Diabetes Mellitus Rats

关键词:  板蓝根水提物  糖尿病  谷丙转氨酶  谷草转氨酶  游离脂肪酸  脂联素  肝组织病理

Key words:  water extract of Radix Isatidis  diabetic  alanine aminotransferase (ALT)  aspartate aminotransferase (AST)  free fatty acids  adiponectin  liver tissue pathology

摘要:

目的 研究板蓝根水提物（water extract of Radix Isatidis，WERI）对糖尿病大鼠肝功能的影响。方法 采用链脲佐菌素诱导Wistar大鼠糖尿病模型，随机分为模型组、罗格列酮组（0.3 mg·kg^-1）、WERI高、中、低剂量组（100，50，25 mg·kg^-1），并设正常对照组，均采用灌胃给药。4周后，检测各组大鼠空腹血糖（FBG）、空腹胰岛素（FINS）、肝系数、谷丙转氨酶（ALT）、谷草转氨酶（AST）、游离脂肪酸（FFA）和血清脂联素（ADP），并观察大鼠肝脏组织病理形态学变化。结果 与正常对照组比较，模型组大鼠FBG、FINS、肝系数、ALT、AST、FFA水平均显著升高（P<0.05或P<0.01）；血清ADP含量显著降低（P<0.05）。与模型组比较，WERI高剂量组大鼠FBG、FINS、肝系数、ALT、AST、FFA水平均显著降低（P<0.05或P<0.01），血清ADP含量升高（P<0.05）；罗格列酮组与WERI高、中、低剂量组大鼠肝脏病理形态均显著改善。结论 WERI对糖尿病大鼠的肝功能具有保护作用。

Abstract:

OBJECTIVE To analysis the influence of water extract of Radix Isatidis(WERI) on liver in diabetic rats.METHODS Diabetic model of Wistar rats induced by streptozotocin. They were randomly divided into model group, rosiglitazone group (0.3 mg·kg^-1), WERI high, medium and low dose group(100, 50, 25 mg·kg^-1). And set up the normal control group, all the use of gastric administration. After 4 weeks, fasting blood glucose (FBG), fasting insulin (FINS), liver coefficient, alanine aminotransferase (ALT), aspartate aminotransferase (AST), free fatty acids (FFA), serum adiponectin (ADP) were measured, and pathological changes in liver tissues of rats were observed.RESULTS Compared with normal control group, FBG, FINS, the liver coefficient, ALT, AST, FFA levels of rats in the model group were increased(P<0.05 or P<0.01); ADP content decreased (P<0.05). Compared with the model group, FBG, FINS, liver coefficient, ALT, AST, FFA levels of WERI high dose group rat were reduced (P<0.05 or P<0.01), ADP content was increased(P<0.05), the difference was statistically significant. The liver of the rosiglitazone group and WERI high, medium and low dose group were significantly improved.CONCLUSION WERI has a protective effect on the liver function of diabetic rats.