| 引用本文: | 赵燕娜,王婷,李然,韩美华,王向涛,郭一飞.新型羟基喜树碱纳米粒形态的影响因素考察[J].中国现代应用药学,2017,34(5):654-658. |
| ZHAO Yanna,WANG Ting,LI Ran,HAN Meihua,WANG Xiangtao,GUO Yifei.Investigation on the Affecting Factors of the Novel Hydroxycamptothecin Nanoparticles Morphology[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(5):654-658. |
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| 新型羟基喜树碱纳米粒形态的影响因素考察 |
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赵燕娜1,2, 王婷1,3, 李然1,3, 韩美华1, 王向涛1, 郭一飞1
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1.中国医学科学院&北京协和医学院药用植物研究所, 北京 100193;2.聊城大学生物制药研究院, 山东 聊城 252059;3.黑龙江中医药大学, 哈尔滨 150040
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| 摘要: |
| 目的 考察制备的新型棒状羟基喜树碱纳米粒形态的影响因素。方法 选择不同投料比、不同制备方法及是否存在其他稳定剂为考察因素，分别进行以新型树枝状大分子PAMAM-co-0.25OEG，PGD为稳定剂的羟基喜树碱纳米粒的制备，并观察纳米粒在电镜下的形态。结果 以PGD为稳定剂的羟基喜树碱纳米粒在电镜下的形态不受制备方法的影响，但和药物与稳定剂的比例，即投料比有关，当投料比从4∶1增加至1∶3时，较多PGD稳定剂的存在更容易包裹药物形成球形纳米粒。Chol-PEG600稳定剂与PGD稳定剂同时存在时，高载药量的羟基喜树碱纳米粒中也会存在球形粒子。结论 羟基喜树碱纳米粒的形态与稳定剂的用量及其结构有关，且稳定剂性质不同，粒径大小相同的纳米粒电位也相差较大。 |
| 关键词: 树枝状大分子 羟基喜树碱 纳米粒 形态 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.05.004 |
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| 基金项目:国家自然科学基金（21444003）；北京协和医学院青年基金（33320140184）；北京协和医学院研究生创新基金（2015-1007-17） |
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| Investigation on the Affecting Factors of the Novel Hydroxycamptothecin Nanoparticles Morphology |
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ZHAO Yanna1,2, WANG Ting1,3, LI Ran1,3, HAN Meihua1, WANG Xiangtao1, GUO Yifei1
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1.Chinese Academy of Medical Sciences and Peking Union Medical College, The Institute of Medicinal Plant Development, Beijing 100193, China;2.Institute of Biopharmaceutical Research, Liaocheng University, Liaocheng 252059, China;3.Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China
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| Abstract: |
| OBJECTIVE To investigate the affecting factors of the morphology on the novel rod hydroxycamptothecin (HCPT) nanoparticles. METHODS Choose different drug loading ratio, different preparation methods and the existence of the other stabilizers or not as examination factors to prepare HCPT nanoparticles stabilized by the novel codendrimer PAMAM-co-0.25OEG, PGD, respectively. The morphology under the electron microscope of the nanoparticles was observed. RESULTS The morphology under the electron microscope of HCPT nanoparticles stabilized by codendrimer PGD was not affected by the preparation methods, but was influenced by the ratio of the drugs and stabilizers (drug loading ratio). While the drug loading ratio increased from 4:1 to 1:3, the existence of large amount of stabilizer PGD made it easier to package HCPT drug and form spherical nanoparticles. HCPT nanoparticles of high drug loading ratio stabilized by both PGD and Chol-PEG600 would also form some spherical particles. CONCLUSION The morphology of HCPT nanoparticles are affected by the radio and structure of the stabilizing agent. HCPT nanoparticles with similar particle size can also be with comparatively different Zeta potential according to the characteristics of the stabilizers. |
| Key words: dendrimer hydroxycamptothecin nanoparticles morphology |
