分散液相微萃取-HPLC测定五味子中五味子甲素和五味子酯甲

Organic Solvent-Based Dispersive Liquid Phase Microextraction Combined with HPLC for Determination of Schizandrin A and Schisantherin A in Schisandra chinensis (Turcz.) Baill.

关键词:  分散液相微萃取  高效液相色谱法  五味子  五味子甲素  五味子酯甲

Key words:  dispersive liquid phase microextraction  HPLC  Schisandra chinensis (Turcz.) Baill.  schizandrin A  schisantherin A

摘要:

目的 采用有机液体分散液相微萃取对五味子药材提取液进行纯化富集，建立分散液相微萃取结合HPLC测定五味子甲素和五味子酯甲的含量。方法 分散液相微萃取以60 μL四氯化碳为萃取剂，供相pH为1，萃取4 min，以转速1500 r.min^-1离心2 min。在上述优化条件下，对五味子药材提取液进行萃取后，以C₁₈柱为固定相、甲醇-0.6%磷酸溶液（69：31）为流动相、检测波长254 nm进行色谱分析。结果 分散液相微萃取对五味子甲素和五味子酯甲的富集倍数分别为5112和1118，线性范围在0.10~19 μg·mL^-1之间，回收率分别为100.1%和94.4%。结论 本法灵敏度高、重现性好，简便、快速、环保，可用于五味子药材中五味子甲素和五味子酯甲的含量测定。

Abstract:

OBJECTIVE To enrich and determine the schizandrin A and schisantherin A in Schisandra chinensis (Turcz.) Baill. by developing an organic solvent-based dispersive liquid phase microextraction combined with HPLC method.METHODS Under the optimal conditions(extraction solvent was CCl₄, the volume of extraction solvent was 60 μL, pH of donor phase was 1 and the extraction time was 4 min, centrifuge at 1 500 r·min^-1 for 2 min), the analytes were extracted. HPLC analyses were performed on a C₁₈ column. The mobile phase consisted of a mixture of methanol and phosphorous acid solution (69:31), and the detection wavelength was set at 254 nm.RESULTS The enrichment factors for schizandrin A and schisantherin A were 5 112 and 1 118, respectively. The linear range was both 0.10-19 μg·mL^-1. And the recoveries of analytes were 100.1% for schizandrin A and 94.4% for schitherin A, respectively.CONCLUSION The method is sensitive, reproductive, simple, rapid and environmental protection, and it can be used to determine schizandrin A and schisantherin A in Schisandra chinensis (Turcz.) Baill..