响应面法优化川丹参总黄酮提取工艺及其体外抗氧化活性评价

Optimization of Extraction of Total Flavonoids from Salvia Miltiorrhiza Bge. by Response Surface Analysis and Evaluation of Its Antioxidant Activity in Vitro

关键词:  川丹参  总黄酮  提取工艺  响应面法  抗氧化

Key words:  Salvia miltiorrhiza Bge.  flavonoids  extraction technology  response surface methodology  antioxidant activity

摘要:

目的 采用响应面法优化川丹参中总黄酮提取工艺，并初步探究总黄酮的体外抗氧化活性。方法 在单因素试验的基础上，以甲醇浓度（A）、提取时间（B）、提取温度（C）为自变量，运用Box-Behnken试验设计-响应面法优化超声辅助提取川丹参总黄酮工艺。同时，测定川丹参总黄酮对超氧自由基（O₂^-·）、羟基自由基（·OH）和DPPH· 3种自由基的体外清除活性。结果 通过响应面试验设计得到川丹参总黄酮最佳提取工艺：甲醇浓度71%，超声时间52 min，超声温度55℃，在此工艺条件下提取得率可达12.493%。在所测浓度范围内，川丹参总黄酮对O₂^-·、·OH和DPPH·均有一定的清除效果，其最大清除率分别达到93.4%，60.3%和40.3%。结论 Box-Behnken设计-响应面法优化川丹参总黄酮提取工艺方法简便，可预测性较好；川丹参总黄酮具有体外抗氧化活性，值得进一步研究和利用。

Abstract:

OBJECTIVE To investigate the ultrasonic-assisted extract on total flavonoids from Salvia miltiorrhiza Bge. and evaluate its in vitro antioxidant activity. METHODS On the basis of single-factor test, the ultrasonic-assisted extraction process parameters of total flavonoids from Salvia miltiorrhiza Bge. were optimized by the response surface method on the methanol concentration(A), extraction time(B) and extraction temperature(C). Meanwhile, the scavenging effects of total flavonoids from Salvia miltiorrhiza Bge. on superoxide free radical(O₂^-·), hydroxyl free radical(·OH) and DPPH· were determined. RESULTS The optimum conditions for total flavonoids of Salvia miltiorrhiza Bge. were as follows:methanol concentration was 71%, ultrasonic time was 52 min, and extraction temperature was 55℃. Under these conditions, the yield of total flavonoids could reach 12.493%. The total flavonoids had a certain scavenging effect on O₂^-·, ·OH and DPPH·, with the maximum scavenging rate of 93.4%, 60.3% and 40.3%, respectively. CONCLUSION The Box-Behnken design and response surface analysis can well optimize the ultrasonic-assisted extraction of total flavonoids from Salvia miltiorrhiza Bge. and has good predictability. The total flavonoids from Salvia miltiorrhiza Bge. have antioxidant activity in vitro, so this plant is worth further research and utilization.