清心饮对柯萨奇B3病毒感染心脏微血管内皮细胞的保护作用

Protective Effects of Qinxin Decoction on Cardiac Microvascular Endothelial Cells Infected by Coxsackievirus B3

关键词:  清心饮  柯萨奇B₃病毒  心脏微血管内皮细胞  内皮细胞转分化

Key words:  Qinxin decoction  Coxsackievirus B3  cardiac microvascular endothelial cells  endothelial-to-mesenchymal transition

摘要:

目的 研究清心饮对柯萨奇B₃病毒（Coxsackievirus B3，CVB₃）感染心脏微血管内皮细胞（cardiac microvascular endothelial cells，CMVECs）的保护作用。方法 采用RT-PCR检测CVB₃ RNA，应用倒置显微镜观察CMVECs形态，CCK8检测细胞活性，选择清心饮最佳干预时间点和稀释浓度。在最佳条件下通过免疫荧光法、Western blot法检测内皮细胞标志物CD31，间充质细胞标志物α-SMA的表达。结果 CVB₃能在CMVECs中持续性复制，清心饮最佳干预时间点和浓度分别是72 h和20%含药血清。与空白组比较，CVB₃组CMVECs形态固缩、变异，CD31表达减弱，α-SMA表达增强；与空白血清组比较，清心饮血清组CMVECs形态趋于正常，CD31表达增强，α-SMA表达减弱。结论 清心饮可抑制CVB₃感染CMVECs发生内皮细胞转分化，对CMVECs具有保护作用。

Abstract:

OBJECTIVE To research the protective effects of Qinxin decoction on CMVECs infected by Coxsackievirus B3 (CVB₃). METHODS CVB₃ RNA were detected by RT-PCR, cells morphology were observed by inverted microscope, cell viability were tested by CCK8 and the best intervention time and concentration of Qinxin decoction were chosen. Then endothelial cells marker-CD31 and mesenchymal cells marker-α-SMA were tested by immunofluorescence and Western blot in optimal conditions. RESULTS CVB₃ could be replicated sustainably in CMVECs. The best intervention time and concentration of Qinxin decoction were 72 h and 20% diluted concentration. Compared with that in the blank group, CD31 protein showed a low expression, but α-SMA showed higher and the cells changed to pyknosis or variation in the group CVB₃. Compared with that in the blank serum group, CD31 protein showed high expression, but α-SMA showed lower and the cells grew quite well in the Qinxin decoction serum group. CONCLUSION Qinxin decoction could inhibit the process of endothelial-to-mesenchymal transition and has a good protective effect on CMVECs following CVB₃ infection.