| 引用本文: | 刘广桢,张媛媛,冷佳蔚,许嘉平,林林.四磨汤合剂中抑菌剂的抑菌效力评价[J].中国现代应用药学,2017,34(2):232-234. |
| LIU Guangzhen,ZHANG Yuanyuan,LENG Jiawei,XU Jiaping,LIN Lin.Evaluation on Antimicrobial Effectiveness of Preservative in Simotang Mixture[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(2):232-234. |
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| 摘要: |
| 目的 对四磨汤合剂中抑菌剂的抑菌效力进行评价。方法 采用HPLC对四磨汤合剂中抑菌剂对羟基苯甲酸乙酯的含量进行测定，并以金黄色葡萄球菌、铜绿假单胞菌、大肠埃希菌、黑曲霉和白色念珠菌为试验菌株，分别加入四磨汤合剂中进行微生物试验，考察在不同时间点样品中微生物的存活情况。结果 四磨汤合剂中对羟基苯甲酸乙酯的含量为0.04%，四磨汤合剂中抑菌剂对细菌的抑制能力较强，而对真菌的抑制能力较弱。结论 四磨汤合剂中抑菌剂的抑菌效力符合中国药典2010年版的要求，不符合中国药典2015年版的要求，现有的抑菌剂处方需进一步优化。 |
| 关键词: 四磨汤合剂 对羟基苯甲酸乙酯 高效液相色谱法 抑菌效力 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.02.017 |
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| Evaluation on Antimicrobial Effectiveness of Preservative in Simotang Mixture |
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LIU Guangzhen, ZHANG Yuanyuan, LENG Jiawei, XU Jiaping, LIN Lin
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Shandong Institute for Food and Drug Control, Jinan 250101, China
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| Abstract: |
| OBJECTIVE To evaluate antimicrobial effectiveness of preservative in simotang mixture.METHODS HPLC was adopted to determinate preservative ethylparaben content of simotang mixture. Using S. aureus, P. aeruginosa, E. coli, A. niger and C. albicans as test strains, the antimicrobial effectiveness test was carried out to evaluate the survival of microorganisms at the different time.RESULTS The concentration of ethylparaben in the sample was 0.04%. Simotang mixture had the strong bacteriostasis and poor fungistasis.CONCLUSION The antimicrobial effectiveness of preservative in simotang mixture meets the requirements of the Ch.P (2010), but does not meet the requirements of the Ch.P (2015). The existing antimicrobial formulations need to be optimized. |
| Key words: Simotang mixture ethylparaben HPLC antimicrobial effectiveness |
