闽产三叶青地上部分提取物体内抗炎镇痛作用研究

Anti-inflammatory and Analgesic Effects of the Extracts of Tetrastigmatis Hemsleyanum's Aerial Parts from Fujian in Vivo

关键词:  三叶青  地上部分  提取物  福建  抗炎  镇痛

Key words:  Tetrastigmatis hemsleyanum  aerial parts  extract  Fujian  anti-inflammatory  analgesic effect

摘要:

目的 研究闽产三叶青地上部分提取物体内抗炎镇痛作用。方法 分别采用低、中、高剂量的闽产三叶青地上部分提取物，抗炎作用实验以二甲苯致小鼠耳肿胀急性炎症模型并以醋酸泼尼松为阳性药、角叉菜胶致大鼠足肿胀急性炎症模型并以醋酸地塞米松为阳性药、大鼠棉球肉芽组织增生慢性炎症模型并以醋酸地塞米松为阳性药，镇痛作用实验以化学刺激法（扭体法）及热刺激法（热板法）并以罗通定为阳性药。结果 与模型组比较，闽产三叶青地上部分提取物中、高剂量组及粗提物组对二甲苯所致小鼠耳廓肿胀有明显的抑制作用（依次为P < 0.05，P < 0.01，P < 0.05），耳肿胀抑制率分别为22.86%，38.79%，20.62%；提取物中、高剂量组及粗提物组对角叉菜胶致大鼠足肿胀急性炎症有明显的抑制作用（P < 0.05或P < 0.01）；提取物低、中、高剂量组及粗提物组均能显著减少大鼠棉球肉芽组织增生慢性炎症模型中大鼠肉芽肿的重量（依次为P < 0.05，P < 0.01，P < 0.01，P < 0.01），其抑制率分别为28.12%，46.41%，59.58%，44.50%。与模型组比较，在60，90 min，提取物高剂量组能有效提高小鼠的痛阈值延长率（P < 0.05，P < 0.01），痛阈值延长率最高达65.58%；提取物中、高剂量组及粗提物组能有效抑制醋酸致痛的小鼠扭体次数（依次为P < 0.05，P < 0.01，P < 0.05），小鼠扭体潜伏期明显延长（依次为P < 0.05，P < 0.01，P < 0.05），扭体次数明显减少（依次为P < 0.05，P < 0.01，P < 0.05），镇痛抑制率最高达51.80%。结论 闽产三叶青地上部分提取物具有明显的体内抗炎镇痛作用。

Abstract:

OBJECTIVE To study the anti-inflammatory and analgesic effects in vivo of extracts of Tetrastigmatis hemsleyanum's aerial parts from Fujian. METHODS High, middle and low dose groups of extracts of Tetrastigmatis hemsleyanum's aerial parts from Fujian were utilized respectively, the anti-inflammatory effects study were developed with models of xylene-induced ear edema in mice of acute inflammation(use prednisone as positive drug), carrageenan-induced paw edema of acute inflammation(use dexamethasone acetate as positive drug) and cotton pellet induced granuloma tissue growth in rat of chronic inflammation(use dexamethasone acetate as positive drug); and the analgesic effects were developed with mice writhing test and mice hot plate test (use rotundine as positive drug). RESULTS Compared with the model group, medium and high dose of extracts of Tetrastigmatis hemsleyanum's aerial parts from Fujian and crude extracts significantly inhibited xylene-induced ear edema in mice(P < 0.05, P < 0.01, P < 0. 05), and the inhibition rates were respectively 22.86%, 38.79%, 20.62%; medium and high dose of Tetrastigmatis hemsleyanum's aerial parts from Fujian and crude extracts could significantly inhibit carrageenan-induced rat paw edema(P < 0.05 and P < 0.01); low, medium and high dose of extracts of Tetrastigmatis hemsleyanum's aerial parts from Fujian and crude extracts could significantly reduced the formation of granulomatous tissue(P < 0.05, P < 0.01, P < 0.01, P < 0.01), and the granuloma inhibition rates were 28.12%, 46.41%, 59.58%, 44. 50%, respectively; compared with the model group, at 60, 90 min, in hot plate test, high dose of extracts of Tetrastigmatis hemsleyanum's aerial parts from Fujian could enhance the extension rate of pain threshold efficiency in mice(P < 0.05, P < 0.01), the maximum of analgesic ratio was 65.58%; and in acetic acid writhing test, medium and high dose of extracts of Tetrastigmatis hemsleyanum's aerial parts from Fujian and crude extracts could significantly inhibit acetic acid-induced writhing pain in mice(P < 0.05, P < 0.01, P < 0.05), and the mice's writhing latency was remarkably prolonged(P < 0.05, P < 0.01, P < 0.05), writhing responses decreased markedly(P < 0.05, P < 0.01, P < 0.05), the maximum of inhibitory ratio was 51.80%. CONCLUSION The extracts of Tetrastigmatis hemsleyanum's aerial parts from Fujian has significant anti-inflammatory and analgesic effect in vivo.