HPLC同时测定小蓟配方颗粒中蒙花苷和芹菜素的含量

Determination of Linarin and Apigenin in Cirsii Herba Dispensing Granule by HPLC

关键词:  小蓟配方颗粒  蒙花苷  芹菜素  高效液相色谱法

Key words:  Cirsii Herba Dispensing Granule  linarin  apigenin  HPLC

摘要:

目的 建立HPLC同时测定小蓟配方颗粒中蒙花苷和芹菜素含量的方法。方法 采用Diamonsil-C₁₈（4.6 mm×250 mm，5 μm）色谱柱，以乙腈-1%冰醋酸水溶液为流动相进行梯度洗脱，流速：1.0 ml·min^-1，检测波长：335 nm，柱温：30℃。结果 蒙花苷和芹菜素进样量分别在0.3~1.5 μg（r=0.999 4）和0.075~0.375 μg（r=0.999 7）内与峰面积呈良好的线性关系；加样回收率分别为99.19%和99.12%，RSD分别为1.27%和1.26%。结论 该方法操作简便，重复性好，可用于小蓟配方颗粒中蒙花苷和芹菜素的含量测定。

Abstract:

OBJECTIVE To develop a method for content determination of linarin and apigenin in Cirsii Herba Dispensing Granule by HPLC. METHODS The separation was carried out on Diamonsil-C₁₈ column (4.6 mm×250 mm,5 μm) under gradient elution with acetonitrile-1% glacial acetic as mobile phase at a flow rate of 1.0 ml·min^-1. The detection wavelength was 335 nm and the column temperature was maintained at 30℃. RESULTS There were good linear relationships between the masses and the peak areas of linarin and apigenin in the ranges of 0.3-1.5 μg (r=0.999 4) and 0.075-0.375 μg (r=0.999 7), respectively. The average recoveries were 99.19% and 99.12%, and RSDs were 1.27% and 1.26%. CONCLUSION The method is simple and reliable for the determination of linarin and apigenin in Cirsii Herba Dispensing Granule.