HPLC测定不同采收期畲药搁公扭根中鞣质类成分含量

Determination of Tannin in Traditional SHE Medicine Rubi Radix et Rhizoma at Different Harvest Time by HPLC

关键词:  搁公扭根  畲药  鞣质  没食子酸  鞣花酸  高效液相色谱法

Key words:  Rubi Radix et Rhizoma  traditional SHE medicine  tannin  gallic acid  ellagic acid  HPLC

摘要:

目的 采用HPLC测定不同月份畲药搁公扭根中鞣质类成分没食子酸和鞣花酸含量，确定其最佳采收期。方法 采用Agilent Zorbax SB-C₁₈柱（4.6 mm×250 mm，5 μm）；流动相为甲醇-0.1%磷酸水溶液（梯度洗脱），流速1.0 mL·min^-1，柱温30℃，检测波长263 nm。结果 不同月份搁公扭根药材中没食子酸含量的差异相对较小，鞣花酸含量的差异较大，但两者在11月-1月期间的含量均较高。结论 搁公扭根以鞣质类成分没食子酸和鞣花酸含量为指标，11月-1月为最佳采收期。

Abstract:

OBJECTIVE To develop an HPLC method for determination of tannin-gallic acid and ellagic acid in traditional SHE medicine Rubi Radix et Rhizoma, and to confirm its best harvest time. METHODS The HPLC was applied with the chromatographic condition as follows:the chromatographic column was Agilent Zorbax SB-C₁₈(4.6 mm×250 mm, 5 μm) with column temperature of 30℃; the mobile phase was a mixed liquid with methanol-0.1% H₃PO₄ solution (gradient elution) at a flow rate of 1.0 mL·min^-1; and the detection wavelength was 263 nm. RESULTS There was no significant difference of the content of gallic acid between different months, while the content of ellagic acid was significantly different, and both content was the highest in November to January. CONCLUSION It is suggested that the best harvest time of Rubi Radix et Rhizoma of tannin (gallic acid and ellagic acid) is in November to January.