4种药用石斛对增强小鼠免疫功能效果的比较研究

Comparison of the Effects of Four Medicinal Species of Dendrobium on Improving Immunological Functions on Mice

关键词:  铁皮石斛  金钗石斛  鼓槌石斛  流苏石斛  免疫功能

Key words:  Dendrobium officinale Kimura et Migo  Dendrobium nobile Lindl.  Dendrobium chrysotoxum Lindl.  Dendrobium fimbriatum Hook.  immune function

摘要:

目的 比较铁皮石斛、金钗石斛、鼓槌石斛和流苏石斛4种药用石斛对增强小鼠免疫功能的效果。方法 4种石斛以0.25，0.50 g·kg^-1连续给予正常小鼠30 d后，测定小鼠的T淋巴细胞增殖能力、巨噬细胞吞噬率和吞噬指数；4种石斛以0.25，0.50 g·kg^-1连续给予受环磷酰胺免疫抑制的小鼠30 d后，显微镜下用细胞计数法计数免疫抑制小鼠血液中的白细胞数、淋巴细胞数和中性粒细胞数。结果 4种石斛0.25，0.50 g·kg^-1剂量均能显著增强T淋巴细胞增殖能力，并显著增加血液中的淋巴细胞数和巨噬细胞吞噬指数；铁皮石斛0.25，0.50 g·kg^-1剂量均能显著提高小鼠巨噬细胞吞噬率、血液中的白细胞数、中性粒细胞数；鼓槌石斛和流苏石斛0.50 g·kg^-1剂量能显著提高小鼠巨噬细胞吞噬率和血液中的白细胞数；金钗石斛0.50 g·kg^-1剂量能显著升高血液中的白细胞数。结论 4种石斛均能增强小鼠免疫功能，但铁皮石斛在增强巨噬细胞吞噬能力及提高免疫抑制小鼠血液中的中性粒细胞数方面效果优于另外3种石斛。

Abstract:

OBJECTIVE To compare the effects of Dendrobium officinale Kimura et Migo(DOK), Dendrobium nobile Lindl.(DNL), Dendrobium chrysotoxum Lindl.(DCL), and Dendrobium fimbriatum Hook.(DFH) on improving the immune functions on mice.METHODS The mice were orally administered with 0.25, 0.50 g·kg^-1 dosage of DOK, DNL, DCL and DFH respectively for 30 d. The T lymphocyte proliferation ability, the phagocytic index and the phagocyticrate of the murine peritoneal macrophage of mice were observed. The immunosuppressed mice induced by cyclophosphamide were orally administered with 0.25, 0.50 g·kg^-1 dosage of DOK, DNL, DCL and DFH respectively for 30 d. The number of leukocyte, lymphocytes and neutrophils in the immunosuppressed mice blood were counted under microscope.RESULTS The T lymphocyte proliferation ability were enhanced, the phagocytic index of the murine peritoneal macrophage and the number of lymphocytes were increased by 0.25, 0.50 g·kg^-1 dosage of DOK, DNL, DCL and DFH. The phagocytic rate, the number of leukocyte and neutrophils in the immune suppressed mice blood were increased by 0.25, 0.50 g·kg^-1 dosage of DOK. The phagocytic rate and the number of leukocyte were increased by 0.50 g·kg^-1 DCL and DFH. The number of leukocyte were increased by 0.50 g·kg^-1 dosage of DNL.CONCLUSION DOK, DNL, DCL and DFH all can enhance the immune functions in mice. DOK is more effective on raising the macrophage phagocytic ability and increasing the number of neutrophils in the immunosuppressed mice blood.