畲药食凉茶HPLC特征图谱和4种成分含量测定

Study on HPLC Characteristic Spectrum of Traditional She Medicine–Shi Liang Cha and Determination of Four Active Components

关键词:  畲药  食凉茶  特征图谱  芦丁  山柰酚-3-O-芸香糖苷  槲皮素  山柰素  含量测定

Key words:  traditional SHE medicine  Shi Liang Cha  characteristic spectrum  rutin  kaempferol-3-O-rutinoside  quercetin  kaempferol  determination

摘要:

目的 采用HPLC建立畲药食凉茶的特征图谱，并同时测定4种成分的含量。方法 以芦丁、山柰酚-3-O-芸香糖苷、槲皮素和山柰素为对照品；采用Agilent Zorbax SB-C₁₈色谱柱（4.6 mm×250 mm，5 μm），柱温30 ℃；乙腈-0.1%磷酸溶液为流动相，梯度洗脱，流速1.0 mL·min^-1；检测波长360 nm。采用中药色谱指纹图谱相似度评价系统对结果进行分析。结果 建立了食凉茶的特征图谱，确定5个共有峰。芦丁、山柰酚-3-O-芸香糖苷、槲皮素和山柰素的线性范围分别为4.525~452.8 μg·mL^-1（r=0.999 9），8.096~809.6 μg·mL^-1（r=1.000 0），0.654~85.15 μg·mL^-1（r=1.000 0），2.048~ 136.6 μg·mL^-1 （r=1.000 0）；平均加样回收率分别为100.51%（n=6，RSD=0.43%），100.19%（n=6，RSD=0.88%），99.98%（n=6，RSD=0.77%），100.26%（n=6，RSD=0.69%）。结论 所建立的特征图谱相关性强，可结合4种成分含量测定全面控制畲药食凉茶的质量，为其规范使用提供科学依据。

Abstract:

OBJECTIVE To establish an HPLC characteristic spectrum of traditional SHE medicine–Shi Liang Cha and simultaneous determination of four active components. METHODS With rutin, kaempferol-3-O-rutinoside, quercetin and kaempferol as reference substance, the HPLC analysis was carried on an Agilent Zorbax SB-C₁₈ column (4.6 mm × 250 mm, 5 μm) with the column temperature of 30 ℃; the mobile phase was acetonitrile-0.1% phosphoric acid solution (gradient elution) at the flow rate of 1.0 mL·min^-1; the detection wavelength was 360 nm. The similarity evaluation system for chromatographic fingerprint of TCM was used to calculate the similar degree. RESULTS The HPLC characteristic spectrum of Shi Liang Cha was established with 5 common peaks. The rutin, kaempferol-3-O-rutinoside, quercetin and kaempferol showed a good linear relationship at the range of 4.525-452.8 μg·mL^-1(r=0.999 9), 8.096-809.6 μg·mL^-1(r=1.000 0), 0.654-85.15 μg·mL^-1(r= 1.000 0), 2.048-136.6 μg·mL^-1(r=1.000 0), respectively; the average recoveries were 100.51%(n=6, RSD=0.43%), 100.19%(n=6, RSD=0.88%), 99.98%(n=6, RSD=0.77%), 100.26%(n=6, RSD=0.69%), respectively.CONCLUSION With a good correlation, the characteristic spectrum can be combined with the assay of 4 components to control the quality of Shi Liang Cha comprehensively, and provide scientific basis for its rational use.