| 引用本文: | 徐烨,俞静静,梁凯伦,陈素红,吕圭源.复方樟脑提取物质量标准研究[J].中国现代应用药学,2017,34(4):496-499. |
| XU Ye,YU Jingjing,LIANG Kailun,CHEN Suhong,LYU Guiyuan.Study on Quality Standard of Compound Camphor Extract[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(4):496-499. |
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| 摘要: |
| 目的 建立复方樟脑提取物的质量标准。方法 采用HPLC对乳香中的11-羰基-β-乙酰乳香酸进行定性鉴别；TLC对没药进行定性鉴别；GC同时测定复方樟脑提取物中的樟脑和冰片的含量。结果 乳香中11-羰基-β-乙酰乳香酸的HPLC色谱峰清晰，分离度好且阴性无干扰；没药TLC斑点清晰，专属性强；樟脑和冰片分别在浓度0.160~0.962 mg·mL-1（r=0.999）和0.246~1.474 mg·mL-1（r=0.999）内线性关系良好，平均回收率分别为102.6%和103.9%。结论 所建标准中定性、定量方法操作简便、准确度高且重现性好，可用于复方樟脑提取物的质量控制。 |
| 关键词: 复方樟脑提取物 质量标准 高效液相色谱法 薄层色谱法 气相色谱法 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.04.004 |
| 分类号: |
| 基金项目:“重大新药创制”国家科技重大专项（2011ZX09101-002-07）；国家自然科学基金资助项目（81274123） |
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| Study on Quality Standard of Compound Camphor Extract |
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XU Ye1, YU Jingjing1, LIANG Kailun1, CHEN Suhong2, LYU Guiyuan1
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1.Zhejiang Chinese Medical University, Hangzhou 310053, China;2.Zhejiang University of Technology, Hangzhou 310053, China
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| Abstract: |
| OBJECTIVE To establish the quality standard for compound camphor extract. METHODS The acetyl-11-keto-β-boswellic acid in frankincense was identified by HPLC. The myrrh was identified by TLC. The content of camphor and synthetic borneol in compound camphor extract were determined by GC. RESULTS The HPLC chromatogram peaks of acetyl-11-keto-β-boswellic acid were clear and well-separated. The clear TLC spots showed good specificity. Camphor and synthetic borneol showed good linear relationships in the range of 0.160-0.962 mg·mL-1 (r=0.999) and 0.246-1.474 mg·mL-1(r=0.999), respectively, with the average recoveries of 102.6% and 103.9%. CONCLUSION The methods are simple, accurate, reproducible and can be used for quality control of compound camphor extract. |
| Key words: compound camphor extract quality standard HPLC TLC GC |
