黄芪与红芪提取物对衰老模型小鼠脑组织抗氧化能力和海马结构功能的影响

Influence of Astragali Radix and Hedysari Radix Extracts on Brain Antioxidant Capability and the Hippocampus Ultrastructure in Aging Model Mice

关键词:  黄芪提取物  红芪提取物  抗氧化  DNA损伤  结构变化

Key words:  Astragali Radix extracts  Hedysari Radix extracts  antioxidant  DNA dammage  morphologic changes

摘要:

目的 研究黄芪、红芪提取物的抗衰老作用。方法 采用D-半乳糖建立小鼠衰老模型，分别灌服同剂量黄芪与红芪提取物进行抗衰老实验。UV检测脑组织T-AOC、SOD活力和MDA含量，单细胞凝胶电泳检测脑细胞DNA损伤。光镜和电镜观察其脑组织学和海马超微结构的改变。结果 黄芪高剂量组，红芪中、高剂量组能显著提高衰老模型小鼠脑组织T-AOC、SOD活性，显著降低MDA含量，明显减轻DNA损伤，明显减轻海马神经元形态结构衰老。结论 黄芪、红芪提取物具有明显的延缓衰老作用，其作用机制可能与提高机体抗氧化能力，减轻自由基对脑细胞DNA的损伤，维护海马区神经细胞的结构完整性，改善或延迟脑组织和神经元退行性变有关。

Abstract:

OBJECTIVE To study the anti-aging activity of Astragali Radix and Hedysari Radix extracts. METHODS The aging mice model was induced by D-glactose. The mice were administered intragastically with same dose of Astragali Radix and Hedysari Radix extracts. The activities of T-AOC, SOD, and the contents of MDA in brain were detected by spectrophotomete; DNA damage of brain cell was observed by single cell gel electrophoresis; morphologic changes of brain histology and hippocampus ultrastructure were observed by microscope and electron microscope. RESULTS The high dose group of Astragali Radix extracts and the middle, high dose group of Hedysari Radix extracts could significantly promote T-AOC, SOD activities, decrease MDA contents in brain, and reduce DNA damage, as well as notably relieved the aging symptoms of morphological structure in hippocampal neurons. CONCLUSION Astragali Radix and Hedysari Radix extracts can delay the aging process, and the possible mechanisms may be due to its enhancement antioxidant capability and relief the DNA damage of brain cell, maintain integrity and postpone degenerative change of hippocampal neurons.