响应面法优化土人参根中总黄酮提取及其抗氧化活性研究

Optimization of Flavonoids Extraction from the Roots of Talinnum Paniculatum by Response Surface Methodology and Study on Its Antioxidant Activity

关键词:  苗族药  土人参  总黄酮  提取  响应面法  抗氧化性

Key words:  traditional miao herbs  Talinum paniculatum  flavonoids  extraction  response surface methodology  antioxidant activity

摘要:

目的 采用不同方法提取苗药用的土人参根中总黄酮，并比较不同提取方法所得总黄酮的抗氧化活性。方法 在单因素试验基础上采用响应面法优化超声波处理后固液比、乙醇体积分数、浸提温度及其交互作用对总黄酮得率的影响，DPPH·、超氧阴离子与羟基自由基清除法考查水浴、超声波辅助和酶辅助提取的苗药土人参根中总黄酮的抗氧化活性。结果 苗药用土人参根中总黄酮的最佳工艺为固液比1︰26、乙醇体积分数65%、浸提温度70 ℃，在此条件下，总黄酮得率为2.620%，与回归方程预测值2.603%符合良好。酶法所得总黄酮的抗氧化活性最强且优于Vc。结论 该优化工艺实现了土人参根中黄酮类化合物的高效提取，不同提取方法得到的总黄酮抗氧化活性差异明显。

Abstract:

OBJECTIVE To compare in vitro antioxidant activity of flavonoids from the Talinnum paniculatum by different extract methods. METHODS On the basis of the single factors, the effects of liquid-solid ratio, ethanol volume fraction and their interaction on extraction of flavonoids were investigated using Box-behnken design response surface method. The antioxidant activities of three methods used to extract the flavoniods: ultrasonic-assisted, water bath and enzymic methods were compared by the experiments, scavenging capabilities of DPPH·free radical, hydroxyl free radical and superoxide aninoic free radical. RESULTS The optimal extraction conditions were as follows: liquid-solid ratio was 26:1, ethanol volume fraction was 65% and extraction temperature was 70 ℃. Under the above conditions, the extraction yield of flavonoids were 2.620%, which was in accord with the predicted values 2.603%. The total flavonoids of Talinnum paniculatum extracted by the enzymic method had the best antioxidant activity and better antioxidant activity than Vc. CONCLUSION The target of extracting flavonoids compound efficiently from Talinum has been achieved by the optimized technology. The flavonids extracted using different methods has the significantly different antioxidant activity.