BioOcularTM角膜上皮模型ET50法在化妆品体外眼刺激评价中的应用

Evaluation of Eye Irritation Potential of Cosmetics Using Human Cornea-like Epithelial Tissue Model BioOcularTM ET50 Method in Vitro

关键词:  三维人源重组角膜  ET₅₀  眼刺激

Key words:  reconstructed human corneal epithelium  ET₅₀  eye irritation

摘要:

目的 通过BioOcular^TM角膜上皮模型ET₅₀法评价不同配方的化妆品在眼刺激性上的强弱。方法 采用三维人源重组角膜上皮模型BioOcular^TM模型ET₅₀法对不同品牌洗发水和面霜的眼刺激性进行比较研究，区分不同配方产品的眼刺激性强弱等级。结果 在同样实验条件下，洗发水和面霜表现出对三维人源重组角膜不同的刺激程度，不同配方品牌的洗发水的刺激程度也有很大差别。结论 采用三维人源重组角膜上皮模型的ET₅₀法是一种评价不同配方化妆品以及原料的眼刺激性的差异的快速替代检测方案。

Abstract:

OBJECTIVE To evaluate eye irritation potential of different shampoos and facial creams using human cornea-like epithelial tissue model BioOcular^TM ET₅₀ method in vitro. METHODS Different shampoos and facial creams were tested in eye irritation ET₅₀ test with reconstructed human cornea-like epithelial tissue model BioOcular^TM in vitro, distinguish eye irritation levels of different formulations. RESULTS Under the same condition, shampoos and facial creams showed irritation at various levels in ET₅₀ test with reconstructed cornea-like epithelial tissue model. Also different ingredients and different brand of shampoos showed different degrees of irritation. CONCLUSION It is a quick alternative way to use ET₅₀ method to evaluate the irritation of different cosmetics and ingredients with reconstructed human cornea-like epithelial tissue model BioOcular^TM in vitro.