大孔树脂纯化胡柚皮总黄酮的工艺研究

Purification of Total Flavonoids from Peels of Citrus Changshan-huyou Y.B.Chang by Macroporous Resin

关键词:  胡柚皮  总黄酮  大孔树脂  纯化工艺  吸附动力学特性

Key words:  peels of Citrus Changshan-huyou Y.B. Chang  total flavonoids  macroporous resin  purification technology  adsorption kinetics characteristics

摘要:

目的 研究胡柚皮总黄酮的大孔树脂纯化工艺。方法 通过对8种不同型号大孔树脂进行静态吸附与解吸实验，优选合适的大孔树脂，并优化分离纯化条件。结果 HPD-300型大孔树脂对胡柚皮总黄酮有较好的吸附和洗脱效果，其最佳分离纯化条件如下：pH 4.0，3.83 mg·mL^-1上样液，上样量为5 mL·g^-1，上样体积流量为2.0 BV·h^-1，依次用2 BV的水洗脱，4 BV 70%乙醇洗脱，洗脱剂的流速为2 BV·h^-1。经HPD-300树脂处理后的总黄酮质量分数达76.21%，收率为93.94%。结论 HPD-300型大孔树脂用于富集胡柚皮总黄酮效果最佳，是一种理想的分离纯化介质。

Abstract:

OBJECTIVE To investigate the purification technology of total flavonoids from the peels of Citrus Changshan-huyou Y.B. Chang by macroporous resin. METHODS Eight macroporous resins were chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters. RESULTS HPD-300 macroporous resin was found to have good adsorption and desorption effects. The optimal purification conditions were pH 4.0, sample mass concentration of 3.83 mg·mL^-1, the loading amount of 5 mL·g^-1, the loading flow rate of 2.0 BV·h-1. The sample was eluted by 2 BV of water and 4 BV of 70% ethanol respectively with the eluting flow rate of 2.0 BV·h-1. The content of total flavonoids increased to 76.21% after the purification, and the yield was 93.94%. CONCLUSION HPD-300 is an ideal resin with satisfactory enrichment for separating and purifying the total flavonoids from peels of Citrus Changshan-huyou Y.B. Chang.