拉坦前列素滴眼液抑菌剂抑菌效力研究

Study on Antimicrobial Effectiveness of Latanoprost Eye Drops

关键词:  拉坦前列素滴眼液  抑菌剂  抑菌效力  苯扎氯铵  羟苯乙酯

Key words:  Latanoprost eye drops  antimicrobial preservative  antimicrobial effectiveness  benzalkonium  ethylparaben

摘要:

目的 探索拉坦前列素滴眼液中抑菌剂的合理添加量。方法 根据药物成分的特征，选择合适的抑菌剂，依照中国药典2015年版抑菌剂效力检查法，采用试验菌株金黄色葡萄球菌、大肠埃希菌、铜绿假单胞菌、白色念珠菌、黑曲霉，对合用防腐剂苯扎氯铵和苯氧乙醇按照不同加入量，配置后进行抑菌效果检查。结果 A、B、C组联合应用防腐剂都能达到规定的抑菌效果。0.03%苯扎氯铵和0.015%羟苯乙酯联合应用防腐剂对上述5种试验菌的生长有良好的抑制作用，且为最低有效浓度。结论 确定0.04%苯扎氯铵和0.03%羟苯乙酯作为拉坦前列素滴眼液的抑菌剂。

Abstract:

OBJECTIVE To explore the reasonable dosage of the antimicrobial agent in Latanoprost eye drops. METHODS According to characteristic of drug ingredients, choose appropriate antimicrobial agents, according to Chinese Pharmacopoeia 2015 Version bacteriostat effect test, the test strains of Staphyococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candia albicans and Aspergillus niger, combination of preservation benzalkonium chloride and ethylparaben according to different join the amount and configuration for antibacterial effect of food inspection. RESULTS A, B, C group combined with the application of preservatives can achieve the prescribed antibacterial effect. 0.03% benzalkonium had a good inhibitory effect on growth of ammonium chloride and 0.015% ethylparaben preservative combined application of the above 5 kinds of test bacteria, and was the lowest effective concentration. CONCLUSION The determination of 0.04% benzalkonium chloride and 0.03% phenoxy ethanol as a bacterciostatic agent of Latanoprost eye drops.