HPLC测定当归中1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol的含量

Determination of the 1-(3',4'-Dihydroxycinnamoyl)-cyclopentane-2,3-diol in Angelicae Sinensis Radix by HPLC

关键词:  当归  高效液相色谱法  1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol  含量测定

Key words:  Angelicae Sinensis Radix  HPLC  1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol  content determination

摘要:

目的 建立HPLC测定当归中1-（3'，4'-dihydroxycinnamoyl）-cyclopentane-2，3-diol含量的方法。方法 采用Inertsil ODS-3 C₁₈柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.05%三氟乙酸水溶液梯度洗脱，流速为1.0 mL·min^-1，检测波长为325 nm，柱温为30℃。结果 1-（3'，4'-dihydroxycinnamoyl）-cyclopentane-2，3-diol进样量在0.023 36~1.168 0 μg（r=1.000 0）内与峰面积呈良好的线性关系，平均回收率为95.33%，RSD为1.88%。结论 该方法简便、准确、重复性好，可为当归药材的质量控制提供参考。

Abstract:

OBJECTIVE To establish an HPLC method for determination of 1-(3',4'-dihydroxycinnamoyl)-cyclopentane -2,3-diol in Angelicae Sinensis Radix. METHODS Inertsil ODS-3 C₁₈ column (4.6 mm×250 mm, 5 μm) was adopted with a mobile phase of acetonitrile-0.05% trifluoroacetic acid at the flow rate of 1.0 mL·min^-1. The detection wavelength was set at 325 nm and the column temperature was 30℃. RESULTS The good linearity with peak areas was achieved in the rang of 0.023 36-1.168 0 μg (r=1.000 0) for 1-(3',4'-dihydroxycinnamoyl)-cyclopentane-2,3-diol. The average recovery was 95.33%, and the RSD was 1.88%. CONCLUSION The method is simple, accurate and reproducible, and can be used for the quality control of Angelicae Sinensis Radix.