HPLC法同时测定跌打丸中3种有效成分的含量

Content Determination of Three Effective Components in Dieda Pills by HPLC

关键词:  跌打丸  芍药苷  川续断皂苷Ⅵ  丹皮酚  高效液相色谱法

Key words:  Dieda pills  paeoniflorin  Asperosaponin  paeonol  HPLC Ⅵ

摘要:

目的 探讨建立同时测定跌打丸中芍药苷、川续断皂苷Ⅵ和丹皮酚含量的方法。方法 采用Agilent ZORBAX C₁₈色谱柱（4.6 mm×250 mm，5 μm）；流速为0.8 mL·min^-1，以乙腈（A）-0.1%磷酸水溶液（B）为流动相，进行梯度洗脱；检测波长为230，212，274 nm。结果 芍药苷进样量在0.514 8~1.544 5 μg与峰面积线性关系良好（r=0.999 7），平均回收率为97.01%（RSD=0.94%，n=6）。川续断皂苷Ⅵ在3.023 8~9.071 4 μg线性关系良好（r=0.999 2），平均回收率为98.44%（RSD=0.26%，n=6）。丹皮酚在0.101 2~0.303 6 μg线性关系良好（r=0.999 8），平均回收率为97.84%（RSD=0.42%，n=6）。结论 建立的方法简便可行，重复性好，灵敏度高，可有效控制跌打丸的质量。

Abstract:

OBJECTIVE To establish the HPLC method for content determination of paeoniflorin, Asperosaponin and Ⅵ paeonol in Dieda pills. METHODS The contents of paeoniflorin, Asperosaponin and paeonol was determined by an HPLC Ⅵ system using following conditions:the column was Agilent ZORBAX C₁₈ (4.6 mm×250 mm, 5 μm) with a mobile phase of acetonitrile(A)-0.1% phosphoric acid(B) in gradient elution mode at the flow rate of 0.8 mL·min^-1, the detection wavelength was at 230, 212 and 274 nm. RESULTS The linear range of paeoniflorin was 0.514 8-1.544 5 μg(r=0.999 7) and its average recovery rates(n=6)were 97.01% (RSD=0.94%). The linear range of Asperosaponin was 3.023 Ⅵ 8-9.071 4 μg(r=0.999 2) and its average recovery rates(n=6)were 98.44%(RSD=0.26%). The linear range of paeonol was 0.101 2-0.303 6 μg(r=0.999 8) and its average recovery rates(n=6)were 97.84%(RSD=0.42%). CONCLUSION The HPLC method is simple, accurate, highly selective and reproducible, thus it can be used as quality control of Dieda pills.