复方当归贝母苦参不同给药方式对早期慢性阻塞性肺病小鼠模型的药效学评价

Evaluation of Chinese Classic Herbal Formula Danggui-beimu-kushen by Aerosol and Oral Administration on Chronic Obstructive Pulmonary Mice Model

关键词:  复方当归贝母苦参  气雾给药  慢性阻塞性肺病

Key words:  formula Danggui-beimu-kushen  aerosol  chronic obstructive pulmonary disease

摘要:

目的 观察复方当归贝母苦参气雾和灌胃给药方式对吸烟致慢阻肺模型小鼠的影响，对其药效进行评价，并对其作用机制进行剖析讨论。方法 收集了肺泡灌洗液（BALF）和肺组织，BALF进行细胞分类计数及细胞因子测定。取肺组织做组织切片进行HE染色，观察炎症细胞状况。取左上肺提取mRNA进行KC、Muc5AC的检测。结果 与模型组对比，复方灌胃和气雾组均能减轻肺组织病理切片上的炎性细胞浸润，其中气雾组较灌胃组缓减轻效果更明显；与模型组相比，灌胃和气雾给药均能显著降低KC、MIP-2含量及Muc5AC的mRNA表达；另外气雾组还可显著降低TNF-α含量及KC的mRNA。结论 复方当归贝母苦参气雾给药较灌胃给药对吸烟模型小鼠的肺部炎症反应、黏液分泌具有更强的抑制作用，其作用机制可能与气雾给药降低TNF-a的含量及KC的mRNA表达更明显有关。

Abstract:

OBJECTIVE To evaluate the effect of different formulation (aerosol and oral) of Chinese classic formula Danggui-beimu-kushen on chronic obstructive pulmonary (COPD) murine model and to study its mechanisms.METHODS The bronchoalveolar lavage fluid (BALF) and lung tissues were collected.Differential WBC count and content of cytokine (KC,MIP2,TNF-α) in BALF,HE staining for lung biopsy,KC and Muc5AC mRNA expression of lung and content in BALF were determined.RESULTS Both the gavage and aerosol groups decreased infiltration of the inflammatory cells than that of the control group,moreover,the aerosol group showed better effect than that of gavage group.The gavage and aerosol groups could further decrease the content of KC,MIP-2 and mRNA expression of Muc5AC.In addition,aerosol group could substantially suppress the content of TNF-a and inhibited mRNA expression of KC.CONCLUSION Aerosol formula showed better effect on the pulmonary inflammation and mucus secretion in COPD murine model than that of gavage formula.The mechanism were involved in decreasing TNF-α content,and mRNA expression of KC.