甘肃紫斑牡丹花粉中脂肪酸的分析研究

Analysis of Fatty Acids in Paeonia Rockii Pollen from Gansu Province

关键词:  紫斑牡丹  花粉  脂肪酸  甲酯化  定量分析  气相色谱-质谱-选择离子监测模式

Key words:  Paeonia rockii  pollen  fatty acid  methyl esterification  quantification  GC-MS-SIM

摘要:

目的 分析评价甘肃紫斑牡丹花粉中的脂肪酸类成分。方法 采用溶剂法超声提取紫斑牡丹花粉中脂肪酸类成分，经三氟化硼-甲醇法催化甲酯化，以GC-MS-SIM对紫斑牡丹花粉及其破壁花粉样品进行分析。采用HP-5MS石英毛细管色谱柱（30 m×0.25 mm，0.25 μm）；载气：高纯氦气；程序升温：起始温度50 ℃，以8 ℃·min^-1升至155 ℃，保持2 min；再以10 ℃·min^-1升至175 ℃，保持2 min；以1.3 ℃·min^-1升至190 ℃，保持8 min；最后以6 ℃·min^-1升至280 ℃，保持5 min。不分流进样；进样口温度：260 ℃；进样量：1 μL。结果 紫斑牡丹花粉中主要含有C8~C24的18种脂肪酸，其中辛酸、肉豆蔻酸、棕榈酸、油酸、亚油酸、γ-亚麻酸含量较高；紫斑牡丹花粉中的脂肪酸以不饱和脂肪酸为主，不饱和脂肪酸占脂肪酸总量的50.45%~73.55%；破壁的紫斑牡丹花粉中脂肪酸总量是未破壁样品的6~7倍。结论 所建立的分析方法简便、准确，适于甘肃紫斑牡丹花粉中脂肪酸类成分的分析。

Abstract:

OBJECTIVE To analyze and evaluate the fatty acids in Paeonia rockii pollen from Gansu province. METHODS The fatty acids in Paeonia rockii pollen collected from Gansu province and it's wall-broken pollen samples were extracted by ultrasonics method with cyclohexane, then methyl esterificated via BF₃-methanol method, and finally detected by GC-MS-SIM. HP-5MS capillary column(30 m×0.25 mm, 0.25 μm) was adopted, and the heating program was 50 ℃ programmed to 155 ℃ at 8 ℃·min^-1 and kept for 2 min, then increased to 175 ℃ at 10 ℃·min^-1 and kept for 2 min, and raised to 190 ℃ at 1.3 ℃·min^-1 and kept for 8 min, finally increased to 280 ℃ at 6 ℃·min^-1 and kept for 5 min. The sample inlet temperature was set at 260 ℃. The injection volume was 1 μL with splitless mode. High purity helium was used as the carrier gas. RESULTS Paeonia rockii pollen contained 18 kinds of fatty acids (C8-C24). The content of caprylic acid, myristic acid, palmitic acid, linoleic acid, oleic acid and γ-linolenic acid were the more higher content among 18 kinds of fatty acids. The fatty acids in Paeonia rockii pollen were comprised of polyunsaturated fatty acids which takes up 50.45%-73.55% of the total fatty acids. The wall-broken pollen was almost 6-7 times as amounts of fatty acids as pollen. CONCLUSION The method is simple, accurate, which can be used for fatty acids analysis in Paeonia rockii pollen.