小通草及其伪品的近红外一致性检验和聚类分析方法研究

Study on Conformity Test and Cluster Analysis of Stachyuri Medulla and Its Adulterants by Near Infrared Spectroscopy

关键词:  小通草  旌节花  青荚叶  西南绣球  近红外漫反射光谱  一致性检验  聚类分析

Key words:  stachyuri medulla  Stachyurus chinensis Franch.  Helwingia japonica (Thunb.) Dietr.  Hydrangea davidii Franch.  near-infraed spectroscopy  conformity test  cluster analysis

摘要:

目的 对小通草及其伪品（西南绣球）进行近红外分析，建立基于近红外漫反射光谱技术的快速、简便的鉴别方法。方法 采用近红外一致性检验和聚类分析方法进行分析，预处理方法为矢量归一加二阶导数法，13点平滑，阈值3.6。结果 在6 202.2~5 199.4 cm^-1、4 701.8~4 196.5 cm^-1谱段内，小通草及其伪品一致性检验、聚类分析结果与性状鉴定结果一致，可以据此进行鉴别。结论 该方法可用于小通草与其伪品的鉴别，并对小通草的质量分析研究有一定意义。

Abstract:

OBJECTIVE To provide a rapid and simple method for identifying Stachyuri Medulla and its adulterants, based on Near-Infrared spectroscopy. METHODS The conformity test mode and cluster analysis mode were established after pre-treatment by the second derivative combining vector normalization method, in which the conformity index limit was set as 3.6 and the smooth points were 13. RESULTS The conformity test and cluster analysis results based on the wavelength range of 6 202.2-5 199.4 cm^-1 and 4 701.8-4 196.5 cm^-1, were coincident with variety identification. The Near-Infrared spectroscopy method could identify stachyuri medulla and its adulterants. CONCLUSION This method can be used for the rapid and accurate diferentiation of them, and may be meaningful for the research on the quality analysis of stachyuri medulla.