| 引用本文: | 黄美霞,洪怀山,廖华军,王英豪,李德森,吴水生.钩吻急性毒性快速检测方法研究[J].中国现代应用药学,2018,35(2):244-247. |
| HUANG Meixia,HONG Huaishan,LIAO Huajun,WANG Yinghao,LI Desen,WU Shuisheng.Rapid Detection Method of Acute Toxicity of Gelsemium Elegans. Benth[J].Chin J Mod Appl Pharm(中国现代应用药学),2018,35(2):244-247. |
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| 摘要: |
| 目的 建立以秀丽隐杆线虫为模式生物的钩吻急性毒性快速筛选方法。方法 将线虫同步化到L4期，优化体系DMSO浓度、吐温-80用量、染毒时间，确定最优实验条件，建立以秀丽隐杆线虫为模式生物的钩吻急性毒性快速筛选方法。结果 以M9缓冲液为基本培养液，秀丽隐杆线虫在钩吻粗提物溶液中暴露72 h的LC50为39.722 mg·mL-1（95%置信限33.802~45.985 mg·mL-1）。结论 该方法简便，快速，试验周期短，样品用量少，该方法可作为钩吻急性毒性研究中钩吻毒性变化的快速判断方法。 |
| 关键词: 钩吻 秀丽隐杆线虫 急性毒性 快速检测 |
| DOI：10.13748/j.cnki.issn1007-7693.2018.02.020 |
| 分类号:R285.5 |
| 基金项目:福建省科技厅资助课题（2015J01329）；福州市食用菌产业发展项目（榕农函[2017]365号） |
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| Rapid Detection Method of Acute Toxicity of Gelsemium Elegans. Benth |
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HUANG Meixia1, HONG Huaishan2, LIAO Huajun1, WANG Yinghao1, LI Desen1, WU Shuisheng1
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1.School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;2.Department of Urology, Fujian Province-owned Hospital/Fujian Provincial Jinshan Hospital, Fuzhou 350028, China
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| Abstract: |
| OBJECTIVE To establish a method for rapid screening of the acute toxicity of Caenorhabditis elegans with Gelsemium elegans. Benth as model organism. METHODS The Caenorhabditis elegans were synchronized to L4 phase. The optimal experimental condition were determined by optimizing the DMSO concentration, Twain-80 dosage and exposure time. A rapid screening method of acute toxicity of Caenorhabditis elegans with Gelsemium elegans. Benth model organisms was established. RESULTS With M9 buffer medium as basic medium, the LC50of Caenorhabditis elegans in gelsemine crude extract solution for 72 h was 39.722 mg·mL-1(95% confidence limit was 33.802-45.985 mg·mL-1). CONCLUSION The method is simple, rapid, short test period, small amount of sample, this method can be used as a rapid method to judge the change in toxicity study on acute toxicity of Gelsemium gelsemium. |
| Key words: Gelsemium elegans. Benth Caenorhabditis elegans acute toxicity rapid detection |
