| 引用本文: | 张文婷,程夏倩,徐靖,黄文康,严爱娟,赵维良.HPLC-ELSD测定灵芝孢子油中甘油三酯的含量[J].中国现代应用药学,2017,34(5):719-722. |
| ZHANG Wenting,CHENG Xiaqian,XU Jin,HUANG Wenkang,YAN Aijuan,ZHAO Weiliang.Determination of Ganoderma Lucidum Spore Oil Triglycerides by HPLC-ELSD[J].Chin J Mod Appl Pharm(中国现代应用药学),2017,34(5):719-722. |
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| HPLC-ELSD测定灵芝孢子油中甘油三酯的含量 |
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张文婷1, 程夏倩2, 徐靖3, 黄文康4, 严爱娟4, 赵维良1
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1.浙江省食品药品检验研究院, 杭州 310052;2.浙江大学医学院附属儿童医院, 杭州 310052;3.浙江省珍稀植物药工程技术研究中心, 武义 321200;4.浙江中医药大学, 杭州 310052
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| 摘要: |
| 目的 采用HPLC-ELSD法研究灵芝孢子油中甘油酯组成。方法 采用ACCHROM Unitary-C18色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-异丙醇（51︰49），流速1.0 mL·min-1，柱温30℃，检测器为蒸发光散射检测器。结果 甘油三亚油酸酯、1，2-二亚油酸-3-油酸甘油酯、1，2-二亚油酸-3-棕榈酸甘油酯、1，2-二油酸-3-亚油酸甘油酯、1-棕榈酸-2-油酸-3-亚油酸甘油酯、甘油三油酸酯、1，2-二油酸-3-棕榈酸甘油酯、1，2-二油酸-3-硬脂酸甘油酯的线性范围分别为0.028 70~0.861 0 μg、0.146 8~4.404 μg、0.0507 5~1.523 μg、0.279 6~8.388 μg、0.242 1~7.263 μg、0.613 2~18.40 μg、0.341 6~10.25 μg、0.0691 5~2.075 μg（r2=0.999 3，0.998 8，0.999 4，0.999 3，0.998 9，0.998 9，0.999 0，0.999 5），加样回收率分别为103.3%，99.0%，103.3%，97.9%，100.1%，104.4%，96.4%和105.0%，RSD分别为4.9%，4.7%，3.8%，5.0%，5.0%，3.4%，4.6%和3.2%。灵芝孢子油中8个甘油酯中相对含量较高的依次是1，2-二油酸-3-棕榈酸甘油酯、甘油三油酸酯、1，2-二油酸-3-亚油酸甘油酯、1-棕榈酸-2-油酸-3-亚油酸甘油酯、1，2-二亚油酸-3-油酸甘油酯，约占8个成分总量的90%。结论 该方法简便、专属性、重复性良好，可为灵芝孢子油的品质鉴定及油脂掺伪鉴别提供理论依据。 |
| 关键词: 灵芝孢子油 高效液相色谱蒸发光散射检测法 甘油三酯 掺伪鉴别 甘油三亚油酸酯 甘油三油酸酯 |
| DOI：10.13748/j.cnki.issn1007-7693.2017.05.019 |
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| Determination of Ganoderma Lucidum Spore Oil Triglycerides by HPLC-ELSD |
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ZHANG Wenting1, CHENG Xiaqian2, XU Jin3, HUANG Wenkang4, YAN Aijuan4, ZHAO Weiliang1
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1.Zhejiang Institute for Food and Drug Control, Hangzhou 310052, China;2.The Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310052, China;3.Zhejiang Province Rare Plant Medicine Engineering Technology Research Center, Wuyi 321200;4.Zhejiang Chinese Medical University, Hangzhou 310052, China
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| Abstract: |
| OBJECTIVE To research the characteristics of Ganoderma lucidum spore oil triglycerides was investigated by HPLC-ELSD. METHODS The chromatographic separation was performed on an ACCHROM Unitary-C18 column (4.6 mm×250 mm, 5 μm) with the mobile phase of acetonitrile-isopropanol(51:49). The flow rate was 1.0 mL·min-1 at 30℃ with the detection by ELSD. RESULTS The calibration curve was linear for trilinolein, 1,2-linolein-3-olein, 1,2-linolein-3-palmitin, 1,2-olein-3-linolein, 1-palmitin-2-olein-3-linolein, triolein, 1,2-olein-3-palmitin and 1, 2-olein-3-stearin in the range of 0.028 70-0.861 0 μg, 0.146 8-4.404 μg, 0.050 75-1.523 μg, 0.279 6-8.388 μg, 0.242 1-7.263 μg, 0.613 2-18.40 μg, 0.341 6-10.25 μg, 0.069 15-2.075 μg (r2=0.999 3, 0.998 8, 0.999 4, 0.999 3, 0.998 9, 0.998 9, 0.999 0 and 0.999 5), respectively. The average recovery was 103.3%, 99.0%, 103.3%, 97.9%, 100.1%, 104.4%, 96.4% and 105.0% with the RSD were 4.9%, 4.7%, 3.8%, 5.0%, 5.0%, 3.4%, 4.6% and 3.2%, respectively. The main components were 1,2-olein-3-palmitin, triolein, 1,2-olein-3-linolein, 1-palmitin-2-olein-3-linolein, 1, 2-linolein-3-olein, accounted for 90% of the total. CONCLUSION The method is rapid, simple and shows good specificity and reproducibility. It can be used for quality control and adulteration analysis of Ganoderma lucidum spore oil. |
| Key words: Ganoderma lucidum spore oil HPLC-ELSD triglyceride adulteration analysis trilinolein triolein |
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