三七总皂苷对TNF-α诱导的肾小球系膜细胞增殖及MMPs/TIMPs表达的影响

Effects of Panax Notoginseng Saponins on TNF-α-induced Proliferation of Mesangial Cells and Expression of MMPs/TIMPs

关键词:  三七总皂苷  肾小球系膜细胞  基质金属蛋白酶  基质金属蛋白酶组织抑制剂

Key words:  Panax notoginseng saponins  Mesangial cells  MMPs  TIMPs

摘要:

目的 观察三七总皂苷（Panax notoginseng saponins，PNS）对TNF-α诱导的小鼠肾小球系膜细胞SV40 MES 13增殖及基质金属蛋白酶/基质金属蛋白酶组织抑制物（MMPs/TIMPs）表达的影响。方法 采用TNF-α诱导SV40 MES 13细胞增殖，xCELLigence RTCA DP实时细胞分析系统记录120 h细胞指数（cell index，CI），实时荧光定量PCR法检测96 h SV40 MES 13细胞MMP-2、TIMP-2mRNA表达，ELISA法检测96 h SV40 MES 13细胞上清MMP-9、TIMP-1表达。结果 PNS 3个剂量组均能抑制TNF-α诱导的SV40 MES 13增殖，其中96 h效果最明显；PNS高、中剂量组能提高MMP-2/TIMP-2比值和MMP-9/TIMP-1比值，与TNF-α组比较差异显著（P<0.05）。结论 PNS能减轻TNF-α诱导的系膜细胞增殖，其机制可能与改善MMP-2/TIMP-2、MMP-9/TIMP-1平衡有关。

Abstract:

OBJECTIVE To study the effect of Panax notoginseng saponins (PNS) on TNF-α-induced proliferation of Mesangial cells and expression of matrix metalloproteinases(MMPs) and tissue inhibitors of metalloproteinases(TIMPs).METHODS Proliferation of SV40 MES 13 cells was induced by TNF-α. Real-time cell analysis system was used to record 120 h cell index (CI). The mRNA level of MMP-2 and TIMP-2 were measured by qRT-PCR. The expression of MMP-9 and TIMP-1 were measured by ELISA. RESULTS All three dose groups of PNS could inhibit the cell proliferation induced by TNF-α, of which 96 h effect was obvious. High, medium dose groups could significantly increase the ratio of MMP-2/TIMP-2 and MMP-9/ TIMP-1 compared with TNF-α group(P<0.05). CONCLUSION PNS can inhibit the proliferation of Mesangial cells induced by TNF-α, and the mechanism may be related to the improvement of MMP-2/TIMP-2, MMP-9/TIMP-1 balance.