FLT3抑制剂CHMFL-FLT3-122解离常数的测定

Determination of Dissociation Constants of FLT3 Inhibitor CHMFL-FLT3-122

关键词:  CHMFL-FLT3-122  解离常数  紫外分光光度法  非对数滴定法

Key words:  CHMFL-FLT3-122  dissociation constants  UV  non-logarithmic titration method

摘要:

目的 测定CHMFL-FLT3-122的2个解离常数。方法 将CHMFL-FLT3-122溶于一系列的不同pH的缓冲溶液中，并在合适的波长处采用UV分别测定吸光度并计算其pK_a1值；以不同比例的乙腈-水（30： 70、40： 60、50： 50、60： 40）为溶剂配制不同浓度（0.009，0.013，0.017 mol·L^-1）的CHMFL-FLT3-122溶液，用盐酸溶液滴定，利用非对数滴定法（Benet法）经数学计算推算出其pK_a2值。结果 CHMFL-FLT3-122存在2个解离常数，其pK_a1值为3.87±0.14，pK_a2值为8.12。结论 采用UV和非对数滴定法对CHMFL-FLT3-122的解离常数进行测定，方法简单快速，准确可靠，可为其制剂的处方设计、质量标准的研究提供参考。

Abstract:

OBJECTIVE To determine the two dissociation constants of CHMFL-FLT3-122.METHODS Dissolve CHMFL-FLT3-122 in a series of different pH values buffer solutions and the absorbance was measured at appropriate wavelengths by UV to calculate the pK_a1.Different proportions of acetonitrile-water mixtures (30:70,40:60,50:50,60:40) were used to prepare different concentrations of CHMFL-FLT3-122 solutions (0.009,0.013,0.017 mol·L^-1) which were titrated by hydrochloric acid solution and the pK_a2 was calculated by non-logarithmic titration method (Benet method).RESULTS The pK_a1 of CHMFL-FLT3-122 was 3.87±0.14 and the pK_a2 of CHMFL-FLT3-122 was 8.12.CONCLUSION The determination of the dissociation constants of CHMFL-FLT3-122 by UV and Non-logarithmic titration method is simple,rapid,accurate and reliable.It can be a reference for the prescription design of preparations and the research of quality standard.